TOXOPLASMA-GONDII - REDISTRIBUTION OF TACHYZOITE SURFACE PROTEIN DURING HOST-CELL INVASION AND INTRACELLULAR DEVELOPMENT
TOXOPLASMA-GONDII - REDISTRIBUTION OF TACHYZOITE SURFACE PROTEIN DURING HOST-CELL INVASION AND INTRACELLULAR DEVELOPMENT
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DOI：
10.1007/bf00931842
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发表时间：
1995-11-01
影响因子：
2
通讯作者：
SMITH, JE
中科院分区：
文献类型：
--
作者：
GRIMWOOD, J;SMITH, JE
免疫过氧化物酶定位的抗原在电子显微镜水平证实，寄生虫的表面蛋白，与膜，脱落的表面上的子虫入侵，而不同量的也内化。SAG 1在细胞内的子孢子上稳定长达48小时，尽管也合成了新的蛋白质。SAG 1存在于子体的表面上，并且在整个感染细胞的不同空泡中发现;这些空泡代表寄生虫空泡的直接延伸或寄生虫表面物质的真正输出。目前尚不清楚该分子是否在细胞内发育过程中继续表达。目前的研究如下SAG 1在入侵过程中的命运，并在第一个48小时内的寄生虫繁殖的宿主细胞，在电子显微镜水平上使用前和后入侵标记技术。
Immunoperoxidase localisation of antigen at the electron microscope level confirms that parasite surface proteins, in association with membrane, are shed from the surface of the zoite on invasion, while varying amounts are also internalised. SAG 1 is stable on intracellular zoites for up to 48 h, although new protein is also synthesised. SAG1 is present on the surface of daughter zoites and is found throughout the infected cell in distinct vacuoles; these vacuoles represent either direct extensions of the parasitophorous vacuole or true export of parasite surface material.Conflicting reports exist concerning the presence of SAG1 on the developing intraphagosomal membrane (IPM) network immediately post-invasion (Sibley et al. 1986; Dubremetz et al. 1993). It is not known whether the molecule continues to be expressed during intracellular development. The current study follows the fate of SAG1 during invasion and over the first 48 h of parasite multiplication within the host cell, using pre- and post-invasion labeling techniques at the electron microscope level.