Differential gene and protein expression between rat tibial nerve and common peroneal nerve during Wallerian degeneration
Differential gene and protein expression between rat tibial nerve and common peroneal nerve during Wallerian degeneration
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华勒变性过程中大鼠胫神经与腓总神经差异基因及蛋白表达
DOI：
10.4103/1673-5374.262602
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发表时间：
2019-12-01
影响因子：
6.1
通讯作者：
Lin, Hao-Dong
中科院分区：
文献类型：
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作者：
Lin, Yao-Fa;Xie, Zheng;Lin, Hao-Dong
Wallerian变性和损伤后的神经再生是涉及许多基因、蛋白质和细胞因子的复杂过程。不同的周围神经损伤后，再生率不同。这是否是由沃勒变性过程中基因和蛋白质的差异表达引起的尚不清楚。将同一大鼠的右侧胫神经和腓总神经暴露并完全切断，然后在同一水平面内缝合。在手术后第1、7、14和21天，从胫神经和腓总神经上切下缝合部位远端1-2 cm的神经组织。然后通过RNA测序和蛋白质组学技术研究损伤神经的沃勒变性过程中基因和蛋白质表达的差异。在胫神经和腓总神经中，分别有1718、1374、1187和2195个差异表达的基因，以及477、447、619和495个差异表达的蛋白质。在沃勒变性过程中激活了47条通路。通过RNA测序显示显著差异表达的三个基因（Hoxd 4、Lpcat 4和Tbx 1）通过实时定量聚合酶链反应进行测定。RNA测序和实时定量聚合酶链反应结果一致。我们的研究结果表明，沃勒氏变性过程中不同时间点受损胫神经和腓总神经中基因和蛋白质的表达存在显着差异。提示不同的周围神经损伤后，其Wallerian变性的生物学过程是不同的。本程序于2016年2月18日获得中国第二军医大学动物实验伦理委员会批准（批准号：CZ 20160218）。
Wallerian degeneration and nerve regeneration after injury are complex processes involving many genes, proteins and cytokines. After different peripheral nerve injuries the regeneration rate can differ. Whether this is caused by differential expression of genes and proteins during Wallerian degeneration remains unclear. The right tibial nerve and the common peroneal nerve of the same rat were exposed and completely cut through and then sutured in the same horizontal plane. On days 1, 7, 14, and 21 after surgery, 1–2 cm of nerve tissue distal to the suture site was dissected out from the tibial and common peroneal nerves. The differences in gene and protein expression during Wallerian degeneration of the injured nerves were then studied by RNA sequencing and proteomic techniques. In the tibial and common peroneal nerves, there were 1718, 1374, 1187, and 2195 differentially expressed genes, and 477, 447, 619, and 495 differentially expressed proteins on days 1, 7, 14, and 21 after surgery, respectively. Forty-seven pathways were activated during Wallerian degeneration. Three genes showing significant differential expression by RNA sequencing (Hoxd4, Lpcat4 and Tbx1) were assayed by real-time quantitative polymerase chain reaction. RNA sequencing and real-time quantitative polymerase chain reaction results were consistent. Our findings showed that expression of genes and proteins in injured tibial and the common peroneal nerves were significantly different during Wallerian degeneration at different time points. This suggests that the biological processes during Wallerian degeneration are different in different peripheral nerves after injury. The procedure was approved by the Animal Experimental Ethics Committee of the Second Military Medical University, China (approval No. CZ20160218) on February 18, 2016.