Application of a Portable Spectrophotometer to Microbial Mat studies: Temperature Dependence of the Distribution of Cyanobacteria and Photosynthetic Bacteria in Hot Spring Water
Application of a Portable Spectrophotometer to Microbial Mat studies: Temperature Dependence of the Distribution of Cyanobacteria and Photosynthetic Bacteria in Hot Spring Water
复制标题
便携式分光光度计在微生物垫研究中的应用：温泉水中蓝藻和光合细菌分布的温度依赖性
DOI：
10.1264/jsme2.2001.255
复制
发表时间：
2001
影响因子：
2.2
通讯作者：
S. Hanada
中科院分区：
文献类型：
--
作者：
M. Sugiura;M. Takano;S. Kawakami;K. Toda;S. Hanada
我们开发了一种简单的吸收分光光度法来鉴定微生物垫上的光合色素，在野外使用便携式分光光度计。该方法可有效地用于光养垫细菌的鉴定和野外混合比例的估算。它还被用来描述长野县中部山温泉开发的温泉微生物垫的结构。微生物群落由蓝藻和绿藻组成，其分布与水温有关。在45~60℃的温度范围内，吸收光谱测定的两种细菌的比例是恒定的，而氯曲霉的光谱混合比例约为40%。这一比率在60至70°C范围内随温度升高而增加；在70°C以上，只观察到柔氯藻。分光光度法还发现了一种粉红色的菌垫，其在801和878 nm处有明显的吸收峰。这些峰强烈地表明该生物体是一个新的分类群的新的光营养细菌。
We developed a simple method for absorbance spectrophotometry to identify photosynthetic pigments of microbial mats using a portable spectrophotometer in the field. This method was very efficient for the identification of mat-forming phototrophs and estimations of their mixing ratios in the field. It was also applied to describe the structure of hot spring microbial mats developed at the Nakabusa Hot Spring, Nagano Prefecture. The microbial mats consisted of cyanobacteria and Chloroflexus, and their distribution depended on the water temperature. The ratio of these two bacteria determined by absorbance spectra was constant at temperatures ranging from 45 to 60°C, and the spectral mixing ratios of Chloroflexus were about 40%. That ratio increased with temperatures in the range of 60 to 70°C; above 70°C, only Chloroflexus was observed. The spectrophotometry also found a pink bacterial mat which had distinctive absorption peaks at 801 and 878 nm. These peaks strongly suggested that the organism was a novel phototrophic bacterium of a new taxon.