Transcription Analysis of the Stress and Immune Response Genes to Temperature Stress in Ostrinia furnacalis
Transcription Analysis of the Stress and Immune Response Genes to Temperature Stress in Ostrinia furnacalis
复制标题
亚洲玉米螟对温度胁迫的应激和免疫反应基因的转录分析
DOI：
10.3389/fphys.2019.01289
复制
发表时间：
2019-10-15
影响因子：
4
通讯作者：
Feng, Congjing
中科院分区：
文献类型：
--
作者：
Chen, Kangkang;Tang, Tai;Feng, Congjing
玉米象是玉米上最重要的害虫之一。在自然环境中，亚洲玉米象幼虫经常受到夏季高温、冬季寒冷等温度挑战。与任何非生物胁迫一样，高温和低温胁迫都会损害昆虫的生理和发育。到目前为止，与高低温胁迫反应相关的基因调控和信号通路的信息还很有限。转录组的高通量测序为检测高温和低温胁迫下的胁迫和免疫反应基因提供了一种新的方法。本研究用8℃和40℃的温度处理亚洲玉米螟幼虫，通过RNA测序和进一步验证，研究了亚洲玉米螟幼虫对温度胁迫的反应。结果表明，在8℃的低温胁迫下，幼虫的免疫应答被上调，而在40℃的低温胁迫下，一些应激反应基因如HSP家族、GST-2、Bax抑制剂和P450的表达显著增加。此外，采用实时定量聚合酶链式反应对免疫相关基因如PGRP-LB、抗菌肽、溶菌酶、丝氨酸蛋白酶和应激反应基因如小分子HSPs和HSP90的表达水平进行了定量，这些基因的表达水平与RNA-Seq结果相似。此外，还发现铁储存蛋白铁蛋白参与了对温度胁迫的反应，血淋巴中总铁浓度的变化与铁蛋白的表达水平大体一致。综上所述，我们的结果表明，应激反应基因参与了对40℃热应激的防御，冷应激引发的免疫反应可能为幼虫提供了对8℃冷应激的保护。更有趣的是，我们的结果表明，在对温度应激的反应中，铁蛋白调节的血淋巴总铁浓度增加，这可能有助于亚洲玉米象在低温和高温胁迫下生存。
Ostrinia furnacalis is one of the most important pests on maize. O. furnacalis larvae are frequently exposed to the temperature challenges such as high temperature in summer and cold temperature in winter in the natural environment. High and low temperature stress, like any abiotic stress, impairs the physiology and development of insects. Up to now, there is limited information about gene regulation and signaling pathways related to the high and cold stress response in O. furnacalis. High-throughput sequencing of transcriptome provides a new approach for detecting stress and immune response genes under high and low temperature stresses in O. furnacalis. In the present study, O. furnacalis larvae were treated with the temperature at 8 and 40°C, and the responses of O. furnacalis larvae to the temperature stress were investigated through RNA-sequencing and further confirmation. The results showed that immune responses were up-regulated in larvae by the cold stress at 8°C while some stress response genes, such as HSP family, GST-2, Bax inhibitor and P450, were significantly increased at 40°C. Furthermore, quantitative real time polymerase chain reaction were performed to quantify the expression levels of immune related genes, such as PGRP-LB, antimicrobial peptides, lysozyme, serine protease and stress response genes such as small HSPs and HSP90, and the expression levels of these genes were similar to the RNA-seq results. In addition, the iron storage protein Ferritin was found to be involved in the response to temperature stress, and the changes of total iron concentration in the hemolymph were, in general, consistent with the expression levels of Ferritin. Taken together, our results suggested that the stress response genes were involved in the defense against the heat stress at 40°C, and the immune responses triggered by cold stress might provide protection for larvae from cold stress at 8°C. More interestingly, our results showed that during the responses to temperature stress, the total iron concentration in hemolymph regulated by Ferritin increased, which might help O. furnacalis in surviving the low and high temperature stress.