Organ distribution of the Ro (SS-A) antigen in the guinea pig.
Organ distribution of the Ro (SS-A) antigen in the guinea pig.
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Ro (SS-A) 抗原在豚鼠体内的器官分布。
DOI：
10.1002/art.1780331209
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发表时间：
1990
影响因子：
--
通讯作者：
Reichlin,M
中科院分区：
文献类型：
--
作者：
Itoh,Y;Kriet,JD;Reichlin,M
使用抗Ro（SS‐ A特异性“夹心”酶联免疫吸附试验（ELISA）和Western免疫印迹法，我们测定了豚鼠盐水灌注器官中的Ro（SS‐A）含量（定量和定性）。所有组织浸提液均含有大量Ro（SS-A）抗原，并可根据夹心ELISA中的定量反应性分为3类。组织提取物中存在的60-kd和52-kd Ro（SS-A）分子形式与Wi-L2提取物中描述的相似，豚鼠红细胞中Ro（SS-A）的54-kd分子形式与人红细胞中发现的相似。蛋白质免疫印迹显示Ro（SS-A）亚型的组织分布在不同组织中不同，对60-kd Ro（SS-A）的反应性与ELISA中观察到的活性相关。与ELISA中高浓度Ro（SS-A）抗原相反，豚鼠肝脏提取物中的60-kd和52-kd Ro（SS-A）条带在Western免疫印迹中非常弱。其他数据表明肝脏中可能存在独特形式的Ro（SS-A）。豚鼠组织有4个Y RNA，相当于HeLa细胞中存在的4个人类RNA-hY 1，hY 3，hY 4和hY 5，而豚鼠红细胞只有一个Y RNA，其大小相当于人类hY 4。Ro（SS-A）抗原的这种异质性表明，不同组织中存在各种Ro（SS-A）基因的差异表达，并扩大了涉及Ro（SS-A）/抗Ro（SS-A）系统的组织特异性病理事件的可能性。
Using an anti—Ro (SS‐A—specific “sandwich” enzyme‐linked immunosorbent assay (ELISA) and Western immunoblotting, we determined the Ro (SS‐A) content (both quantitative and qualitative) in saline‐perfused organs of the guinea pig. All tissue extracts contained substantial concentrations of Ro (SS‐A) antigen and could be grouped into 3 categories based on quantitative reactivity in the sandwich ELISA. The 60‐kd and 52‐kd molecular forms of Ro (SS‐A) present in tissue extracts were similar to those described in Wi‐L2extracts, and the 54‐kd molecular form of Ro (SS‐A) in guinea pig erythrocytes was similar to that found in human erythrocytes. The tissue distribution of the isoforms of Ro (SS‐A) was shown by Western immunoblotting to vary in different tissues, and the reactivity to the 60‐kd Ro (SS‐A) was correlated with the activity seen in the ELISA. Both the 60‐kd and 52‐kd Ro (SS‐A) bands in guinea pig liver extracts were very weak on Western immunoblots, in contrast to the high concentration of Ro (SS‐A) antigen in the ELISA. Other data suggest the possible existence of a unique form of Ro (SS‐A) in the liver. Guinea pig tissues have 4 Y RNA that are equivalent to the 4 human RNA—hY1, hY3, hY4, and hY5—present in HeLa cells, while guinea pig red blood cells have only one Y RNA, which is equivalent in size to human hY4. This heterogeneity of the Ro (SS‐A) antigen suggests that there is differential expression of the various Ro (SS‐A) genes in different tissues and expands the possibilities for tissue‐specific pathologic events involving the Ro (SS‐A)/anti—Ro (SS‐A) system.