Immunocytochemical localization of relaxin in human corpora lutea: cellular and subcellular distribution and dependence on reproductive state.
Immunocytochemical localization of relaxin in human corpora lutea: cellular and subcellular distribution and dependence on reproductive state.
复制标题
人黄体中松弛素的免疫细胞化学定位：细胞和亚细胞分布以及对生殖状态的依赖性。
DOI：
10.1095/biolreprod44.6.1140
复制
发表时间：
1991
影响因子：
3.6
通讯作者：
Sanfilippo,J
中科院分区：
文献类型：
--
作者：
Stoelk,E;Chegini,N;Lei,ZM;Rao,CV;Bryant-Greenwood,G;Sanfilippo,J
松弛素是妊娠期间存在的激素之一，主要由黄体（CL）合成。其他生殖组织，包括月经周期的CL也可以合成这种激素。然而，关于松弛素在人类CL中的细胞和亚细胞分布以及黄体松弛素对生殖状态的依赖性知之甚少。本文所述的光镜和电镜免疫细胞化学研究是利用猪和人松弛素的抗血清获得的。在大黄体细胞（17-30 μM）中发现免疫染色，但在小黄体细胞（7-16 μM）或任何生殖状态的非黄体细胞或人肝细胞中未发现免疫染色。黄体免疫染色在黄体早期较低，在黄体晚期逐渐增加，达到最高水平，然后在白色体中显著下降。足月妊娠CL包含类似的免疫染色早期黄体期CL。中期黄体期CL含有更多的免疫染色细胞比晚期黄体期CL，但晚期黄体期CL含有更大量的免疫染色比中期黄体期CL每个细胞。由于松弛素的免疫金颗粒主要存在于分泌颗粒和在粗面内质网的一小部分。定量分析显示，分泌颗粒含有大量的金颗粒比粗面内质网。这两个细胞器中的晚期黄体CL比中期黄体含有更多的金颗粒。少量含有金颗粒的分泌颗粒和囊泡存在于质膜附近，表明在黄体中期和晚期CL中几乎没有胞吐作用。总之，松弛素的免疫染色存在于所有生殖状态的人CL中，其中最大量存在于黄体晚期CL中。只有大的黄体细胞含有这种激素，具有典型分泌蛋白的亚细胞分布。
Relaxin is one of the hormones present during pregnancy and it is synthesized primarily by corpora lutea (CL). Other reproductive tissues including CL of the menstrual cycle may also synthesize this hormone. Very little is known, however, about the cellular and subcellular distribution of relaxin in human CL and dependence of luteal relaxin on the reproductive state. The light and electron microscope immunocytochemical studies described here were undertaken to obtain this information using antisera to porcine and human relaxin. Immunostaining was found in large luteal cells (17–30 μM) but not in small luteal cells (7–16 μM) or in nonluteal cells in any of the reproductive states or in human hepatocytes. Luteal immunostaining was low in early luteal phase; it increased progressively, reaching the highest level in late luteal phase, and then decreased greatly in corpora albicantia. Term pregnancy CL contained similar immunostaining as early luteal phase CL. Mid luteal phase CL contained more immunostained cells than late luteal phase CL, but the late luteal phase CL contained a greater amount of immunostaining per cell than mid luteal phase CL. The immunogold particles due to relaxin were primarily present in secretory granules and to a small extent in rough endoplasmic reticulum. Quantitation revealed that secretory granules contained a much higher number of gold particles than did rough endoplasmic reticulum. These two organelles from late luteal phase CL contained greater numbers of gold particles than those from mid luteal phase. A small number of secretory granules and vesicles containing gold particles were present adjacent to plasma membranes, indicating little exocytosis in mid and late luteal phase CL In conclusion, immunostaining for relaxin is present in human CL of all the reproductive states, with the greatest amount present in late luteal phase CL Regardless of the reproductive state, only large luteal cells contained this hormone with the subcellular distribution of a typical secretory protein.