Global transcriptional responses of fission yeast to environmental stress
Global transcriptional responses of fission yeast to environmental stress
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DOI：
10.1091/mbc.e02-08-0499
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发表时间：
2003-01-01
影响因子：
3.3
通讯作者：
Bähler, J
中科院分区：
文献类型：
--
作者：
Chen, DR;Toone, WM;Bähler, J
我们探讨了裂殖酵母粟酒裂殖酵母对各种环境胁迫的转录反应。DNA微阵列被用来表征所有已知的和预测的基因表达谱的变化，响应于五种胁迫条件：氧化应激引起的过氧化氢，重金属胁迫引起的镉，热休克引起的温度升高到39 degreesC，渗透胁迫引起的山梨醇，和DNA损伤引起的烷化剂甲基甲烷磺酸。我们定义了一个共同的核心环境压力反应（CESR）的所有，或大多数，压力。有大量的重叠CESR基因的裂殖酵母和芽殖酵母的基因，是刻板的调节过程中的压力。CESR基因主要由应激激活的丝裂原活化蛋白激酶Sty1p和转录因子Atf1p控制。S.粟酒裂殖酵母还激活了对给定的胁迫或胁迫子集更加专门化的基因表达程序。在一般情况下，这些“应激特异性”反应较少依赖于Sty1p丝裂原活化蛋白激酶途径，并可能涉及特定的调节因子。与一些组的共调节基因的启动子基序进行了鉴定。我们比较和对比裂变和芽殖酵母的应激基因的全球调节，并讨论进化的影响。
We explored transcriptional responses of the fission yeast Schizosaccharomyces pombe to various environmental stresses. DNA microarrays were used to characterize changes in expression profiles of all known and predicted genes in response to five stress conditions: oxidative stress caused by hydrogen peroxide, heavy metal stress caused by cadmium, heat shock caused by temperature increase to 39degreesC, osmotic stress caused by sorbitol, and DNA damage caused by the alkylating agent methylmethane sulfonate. We define a core environmental stress response (CESR) common to all, or most, stresses. There was a substantial overlap between CESR genes of fission yeast and the genes of budding yeast that are stereotypically regulated during stress. CESR genes were controlled primarily by the stress-activated mitogen-activated protein kinase Sty1p and the transcription factor Atf1p. S. pombe also activated gene expression programs more specialized for a given stress or a subset of stresses. In general, these "stress-specific" responses were less dependent on the Sty1p mitogen-activated protein kinase pathway and may involve specific regulatory factors. Promoter motifs associated with some of the groups of coregulated genes were identified. We compare and contrast global regulation of stress genes in fission and budding yeasts and discuss evolutionary implications.