Preparation of laminin/nidogen adsorbed urinary bladder decellularized materials via a mussel- inspired polyd
Preparation of laminin/nidogen adsorbed urinary bladder decellularized materials via a mussel- inspired polyd
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仿贻贝聚合法制备层粘连蛋白/巢蛋白吸附的膀胱脱细胞材料
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发表时间：
2017
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通讯作者：
Yong Huang
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作者：
Liangpeng Ge;Chuan Cao;Shixuan Chen;Bin Shao;Qianyong Li;Qingtao Li;Lubin Liu;Zhiqing Liang;Yong Huang
盆腔器官脱垂（POP）是一个严重的健康问题，影响许多成年女性。对于脱垂的盆腔器官，常用的重建方法是使用支架材料重建脱垂的盆腔器官。然而，现有的骨盆重建材料在生物相容性、力学和免疫排斥方面不符合临床要求。为了解决这些问题，选择膀胱脱细胞材料（UBDM），因为它们具有良好的应变-应力抗性。为了增强其生物相容性，使用层粘连蛋白/巢蛋白用贻贝启发的聚多巴胺涂层修饰UBDM。我们发现层粘连蛋白/巢蛋白-多巴胺-UBDM的生物相容性和力学性能显著增强，层粘连蛋白/巢蛋白-多巴胺-UBDM的降解速率显著降低。此外，层粘连蛋白/巢蛋白-多巴胺-UBDM组中CD 31的表达高于正常UBDM组。层粘连蛋白/巢蛋白-多巴胺-UBDM治疗主要引导M2型巨噬细胞并导致炎症反应。这些结果表明层粘连蛋白/巢蛋白吸附的膀胱脱细胞材料有希望用于骨盆重建。
Pelvic organ prolapse (POP) is a serious health issue that affects many adult women. A common strategy .for POP reconstruction is to use scaffold materials to reconstruct the prolapsed pelvic organ. However, the existing .materials for pelvic reconstruction do not meet the clinical requirements for biocompatibility, mechanics and immunological rejection. To address these concerns, urinary bladder decellularized materials (UBDM) was selected .due to their good strain-stress resistance. To enhance its biocompatibility, laminin/nidogen was used to modify the .UBDM with a mussel-inspired polydopamine coating. We found that the biocompatibility and mechanical properties .of laminin/nidogen-Dopamine-UBDM were significantly enhanced and that the degradation rate of laminin/nidogenDopamine-UBDM was markedly reduced. Moreover, the expression of CD31 in the laminin/nidogen-Dopamine-UBDM group was higher than that in the normal UBDM group. The laminin/nidogen-Dopamine-UBDM treatment mainly .guided M2 type macrophages and led to an inflammatory response. These results indicate that laminin/nidogen .adsorbed urinary bladder decellularized materials are promising for use in pelvic reconstruction.