Distribution of Prolactin-Responsive Neurons in the Mouse Forebrain
Distribution of Prolactin-Responsive Neurons in the Mouse Forebrain
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DOI：
10.1002/cne.22208
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发表时间：
2010-01-01
影响因子：
2.5
通讯作者：
Grattan, David R.
中科院分区：
文献类型：
--
作者：
Brown, Rosemary S. E.;Kokay, Ilona C.;Grattan, David R.
催乳素在大脑中具有许多生物学作用，转基因小鼠越来越多地被用于研究这些作用。本研究旨在通过原位杂交描述催乳素受体mRNA的分布，并通过免疫组织化学测量催乳素诱导的信号转导和转录激活因子5（pSTAT 5）的磷酸化，提供一个详细的映射催乳素反应神经元在雌性小鼠前脑。对于原位杂交，设计用于检测长和短受体亚型的探针显示前脑内以异质方式表达mRNA。在下丘脑吻侧，特别是脑室周围区域，以及下丘脑内侧基底核的弓状核和腹内侧核中观察到强表达。在一些非下丘脑区域也有显著表达，特别是在脉络丛中的高表达，而在内侧杏仁核、终纹床核和外侧隔中的表达水平较低。通过免疫组织化学检测，催乳素诱导的pSTAT 5提供了神经元中催乳素受体激活的功能指标。催乳素诱导的pSTAT 5仅在含有催乳素受体mRNA的区域中观察到，并且在吻侧和内侧基底下丘脑中特别突出。大多数含有催乳素受体mRNA的其他区域也显示催乳素诱导的pSTAT 5阳性信号。主要的例外是室旁核和正中视前核，其中观察到催乳素受体mRNA，但没有诱导pSTAT 5催乳素。这些数据提供了关键的神经解剖学信息，促进了小鼠模型的使用，以进一步了解催乳素在大脑中的作用。神经学比较杂志518：92-102，2010. (C)2009 Wiley-Liss，Inc.
Prolactin has numerous biological actions in the brain, and transgenic mice are increasingly being used to investigate these actions. The present study aimed to provide a detailed mapping of the prolactin-responsive neurons in the female mouse forebrain by describing the distribution of prolactin receptor mRNA by in situ hybridization, and measuring prolactin-induced phosphorylation of signal transducer and activation of transcription 5 (pSTAT5) by immunohistochemistry. For in situ hybridization, a probe designed to detect both long and short receptor isoforms showed mRNA expression in a heterogeneous manner within the forebrain. Strong expression was observed in the rostral hypothalamus, particularly in periventricular regions, as well as in the arcuate and ventromedial nuclei of the mediobasal hypothalamus. There was also significant expression in some nonhypothalamic regions, notably high expression in the choroid plexus, and lower levels of expression in the medial amygdala, bed nucleus of the stria terminalis, and lateral septum. Prolactin-induced pSTAT5, detected by immunohistochemistry, provided a functional index of prolactin receptor activation in neurons. Prolactin-induced pSTAT5 was only observed in areas containing prolactin receptor mRNA, and was particularly prominent in the rostral and mediobasal hypothalamus. Most other areas that contained prolactin receptor mRNA also showed positive signal for prolactin-induced pSTAT5. The major exceptions were paraventricular nucleus and median preoptic nucleus, in which prolactin receptor mRNA was observed, but no induction of pSTAT5 by prolactin. The data provide key neuroanatomical information facilitating the use of the mouse model for furthering our understanding of prolactin actions in the brain. J. Comp. Neurol. 518:92-102, 2010. (C) 2009 Wiley-Liss, Inc.