Critical fluctuations in plasma membrane vesicles
Critical fluctuations in plasma membrane vesicles
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DOI：
10.1021/cb800012x
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发表时间：
2008-05-01
影响因子：
4
通讯作者：
Baird, Barbara
中科院分区：
文献类型：
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作者：
Veatch, Sarah L.;Cicuta, Pietro;Baird, Barbara
我们证明了直接从活细胞中分离的巨质膜囊泡（GPMV）的关键行为。GPMV在低温下含有两个液相，在高温下含有一个液相，并且表现出在15至25摄氏度范围内的转变温度。在两相区域中，随着温度升高至转变，线张力线性接近零。在单相区，微米级的组成波动发生，并变得越来越大，寿命长的温度降低到过渡。这些结果表明接近一个临界点，并与建立的理论定量一致。我们观察到的强临界波动表明，哺乳动物质膜的组成被调整到接近一个临界点，
We demonstrate critical behavior in giant plasma membrane vesicles (GPMVs) that are isolated directly from living cells. GPMVs contain two liquid phases at low temperatures and one liquid phase at high temperatures and exhibit transition temperatures in the range of 15 to 25 degrees C. In the two-phase region, line tensions linearly approach zero as temperature is increased to the transition. In the one-phase region, micrometer-scale composition fluctuations occur and become increasingly large and long-lived as temperature is decreased to the transition. These results indicate proximity to a critical point and are quantitatively consistent with established theory. Our observations of robust critical fluctuations suggest that the compositions of mammalian plasma membranes are tuned to reside near a miscibility critical point and that heterogeneity corresponding to