Revisiting the housekeeping genes of human placental development and insufficiency syndromes
Revisiting the housekeeping genes of human placental development and insufficiency syndromes
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DOI：
10.1016/j.placenta.2012.09.007
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发表时间：
2012-11-01
期刊：
影响因子：
3.8
通讯作者：
Kingdom, J.
中科院分区：
文献类型：
--
作者：
Drewlo, S.;Levytska, K.;Kingdom, J.
半定量RT-PCR基因表达分析是胎盘研究中常用的工具。然而，不同临床组之间的稳态基因转录的比较依赖于目标mRNA数据与从所谓的管家(HK)基因获得的mRNA的比较，这些基因的稳态转录在组之间没有显着差异。在这次交流中，我们评估了胎盘研究中常用的九个临床组候选HK基因的表现。我们使用GeNorm方法对qRT-PCR数据进行评估以确定候选HKS的性能。(C)2012爱思唯尔有限公司。保留所有权利。
Gene expression analysis using semi-quantitative RT-PCR is a common tool in placental research. However the comparison of steady-state gene transcription between different clinical groups is dependent upon comparison of target mRNA data with mRNA obtained from so-called housekeeping (HK) genes whose steady-state transcription does not differ significantly between the groups. In this communication, we evaluated the performance of candidate HK genes across nine clinical groups commonly used in placental research. We used the GeNorm method to evaluate qRT-PCR data to determine the performance of candidate HKs. (c) 2012 Elsevier Ltd. All rights reserved.