The heterologous expression of a chrysanthemum TCP-P transcription factor CmTCP14 suppresses organ size and delays senescence in Arabidopsis thaliana
The heterologous expression of a chrysanthemum TCP-P transcription factor CmTCP14 suppresses organ size and delays senescence in Arabidopsis thaliana
复制标题
菊花 TCP-P 转录因子 CmTCP14 的异源表达抑制拟南芥器官大小并延迟衰老
DOI：
10.1016/j.plaphy.2017.03.026
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发表时间：
2017
影响因子：
6.5
通讯作者：
Chen FD
中科院分区：
文献类型：
--
作者：
Zhang Ting;Qu Yixin;Wang Haibin;Wang Jingjing;Song Aiping;Hu Yueheng;Chen Sumei;Jiang Jiafu;Chen Fadi;Chen FD
TCP转录因子在植物生长发育中起着重要作用，但其在菊花中的活性尚未得到深入研究。在这里，菊花TCP-P序列，它编码一种蛋白质窝藏保守的基本螺旋-环-螺旋（bHLH）基序，被证明是相关的拟南芥基因AtTCP 14。酵母单杂交实验表明，编码蛋白不具有转录激活能力，定位实验表明其定位于细胞核内。转录谱确定该基因在茎和叶中最活跃。它在拟南芥中的异源表达可调节某些细胞周期相关基因，缩小各种器官的大小，增加叶片叶绿素和类胡萝卜素含量，从而延缓衰老，延长花期。通过对菊花cDNA文库的筛选，发现CmTCP 14可以与CmFTL 2和部分CmDELLA相互作用。
TCP transcription factors are important for plant growth and development, but their activity in chrysanthemum (Chrysanthemum morifolium) has not been thoroughly explored. Here, a chrysanthemum TCP-P sequence, which encodes a protein harboring the conserved basic helix-loop-helix (bHLH) motif, was shown to be related phylogenetically to the Arabidopsis thaliana geneAtTCP14. A yeast-one hybrid assay showed that the encoding protein had no transcriptional activation ability, and a localization experiment indicated that it was localized in the nucleus. Transcription profiling established that the gene was most active in the stem and leaf. Its heterologous expression inA. thalianadown-regulated certain cell cycle-related genes, reduced the size of various organs and increased the chlorophyll and carotenoid contents of the leaf which led to delayed senescence and a prolonged flowering period. Moreover, by screening the cDNA library of chrysanthemum, we found that the CmTCP14 can interact with CmFTL2 and some CmDELLAs.