Cryptochrome 3 from Arabidopsis thaliana:: Structural and functional analysis of its complex with a folate light antenna
Cryptochrome 3 from Arabidopsis thaliana:: Structural and functional analysis of its complex with a folate light antenna
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DOI：
10.1016/j.jmb.2006.11.066
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发表时间：
2007-02-23
影响因子：
5.6
通讯作者：
Essen, Lars-Oliver
中科院分区：
文献类型：
--
作者：
Klar, Tobias;Pokorny, Richard;Essen, Lars-Oliver
Cryptochromes are almost ubiquitous blue-light receptors and act in several species as central components of the circadian clock. Despite being evolutionary and structurally related with DNA photolyases, a class of light-driven DNA-repair enzymes, and having similar cofactor compositions, cryptochromes lack DNA-repair activity. Cryptochrome 3 from the plant Arabidopsis thaliana belongs to the DASH-type subfamily. Its crystal structure determined at 1.9 angstrom resolution shows cryptochrome 3 in a dimeric state with the antenna cofactor 5,10-methenyltetrahydrofolate (MTHF) bound in a distance of 15.2 angstrom to the U-shaped FAD chromophore. Spectroscopic studies on a mutant where a residue crucial for MTHF-binding, E149, was replaced by site-directed mutagenesis demonstrate that MTHF acts in cryptochrome 3 as a functional antenna for the photoreduction of FAD. (c) 2006 Elsevier Ltd. All rights reserved.
