Glycolipid presentation to natural killer T cells differs in an organ-dependent fashion
Glycolipid presentation to natural killer T cells differs in an organ-dependent fashion
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DOI：
10.1073/pnas.0408288102
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发表时间：
2005-01-25
影响因子：
11.1
通讯作者：
Tsuji, M
中科院分区：
文献类型：
--
作者：
Schmieg, J;Yang, GG;Tsuji, M
已经证明树突状细胞（dc）能够在体内向自然杀伤（NK） T细胞提供糖脂。然而，树突状细胞的基本作用以及其他细胞在糖脂递呈中的作用尚不清楚。在这里，我们发现dc是脾脏NK T细胞的关键抗原呈递细胞（APCs），而Kupffer细胞是肝脏NK T细胞的关键APCs。两种细胞类型在糖脂给药后2小时内刺激NK T细胞产生细胞因子，但只有dc参与糖脂给药的全身下游反应。更具体地说，CD8alpha+ dc在糖脂呈递下产生IL-12，刺激不同器官NK细胞产生次生ifn - γ。不同的apc在体内参与糖脂向NK T细胞的递呈，但参与整体糖脂反应的程度不同。
It has been shown that dendritic cells (DCs) are able to present glycolipids to natural killer (NK) T cells in vivo. However, the essential role of DCs, as well as the role of other cells in glycolipid presentation, is unknown. Here, we show that DCs are the crucial antigen-presenting cells (APCs) for splenic NK T cells, whereas Kupffer cells are the key APCs for hepatic NK T cells. Both cell types stimulate cytokine production by NK T cells within 2 h of glycolipid administration, but only DCs are involved in the systemic, downstream responses to glycolipid administration. More specifically, CD8alpha+ DCs produce IL-12 in response to glycolipid presentation, which stimulates secondary IFN-gamma production by NK cells in different organs. Different APCs participate in glycolipid presentation to NK T cells in vivo but differ in their involvement in the overall glycolipid response.