Elaboration and evaluation of a new screening medium for detection and presumptive identification of extended-spectrum beta-lactamase-producing organisms (ESBL)
Elaboration and evaluation of a new screening medium for detection and presumptive identification of extended-spectrum beta-lactamase-producing organisms (ESBL)
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用于检测和推定鉴定超广谱 β-内酰胺酶产生生物体 (ESBL) 的新型筛选介质的制定和评估
DOI：
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发表时间：
2000
期刊：
影响因子：
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通讯作者：
Carlos Henrique Pessôa de Menezes e Silva
中科院分区：
文献类型：
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作者：
Carlos Henrique Pessôa de Menezes e Silva
新的s-内酰胺酶的出现主要是由于大量使用新的广谱s-内酰胺抗生素。卫生专业人员需要了解新酶引起的耐药性问题，并了解检测、预防和控制此类问题的正确程序。本研究采用头孢他啶-肌醇-万古霉素-两性霉素B琼脂（Ceftazidime-Inositol-Vancomycin-Amphotericin B Agar，CIVA）筛选产ESBL肠杆菌科细菌，并对其微生物学性能进行评价。在Santa Monica医院（Vila Velha，ES，Brazil）住院患者的126份粪便样本中进行，这些患者严重暴露于广谱抗生素组合。基于菌落颜色（由于肌醇发酵），通过培养基检测细菌。为了正确鉴定这些菌株，需要进行额外的试验。未检测到假阳性率。
The new s-lactamases have arisen largely as a consequence of heavy use of new expanded spectrum s-lactam antibiotics. Health professionals need to be aware of the resistance problems caused by the new enzymes, and know the correct procedures to detect, prevent and control such problems. In this study, a new screening medium called Ceftazidime-Inositol-Vancomycin-Amphotericin B Agar (CIVA) was elaborated and the microbiological performance was evaluated for the detection and presumptive identification of ESBL-producing members of Enterobacteriaceae. It was performed in 126 stool samples from hospitalized patients at Santa Monica Hospital (Vila Velha, ES, Brazil), who had been heavily exposed to broad-spectrum antibiotic combinations. The bacteria were detected by the medium based on their colony colours (due to inositol fermentation). Additional tests were required for correct identification of these strains. No false positive rates were detected.