Surface modification of MCM-41 and its application in DNA adsorption
Surface modification of MCM-41 and its application in DNA adsorption
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DOI：
10.1016/j.cclet.2015.08.008
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发表时间：
2016
影响因子：
9.1
通讯作者：
Xiuyu Sun;Peng Li;Bing Ai;Yue‐Bo Wang
中科院分区：
文献类型：
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作者：
Xiuyu Sun;Peng Li;Bing Ai;Yue‐Bo Wang
通过胺接枝、膦酸盐改性和金属离子螯合制备了Al3+ -MCM-41、La3+ -MCM-41和Zn2+ -MCM-41三种类型的MCM-41吸附剂，并通过傅里叶变换红外光谱、热重分析和n2吸附-解吸分析对其进行了表征。结果表明，功能化后的MCM-41保持了分子筛的原有结构，孔径和比表面积均比纯MCM-41减小。根据硬-软酸碱（HSAB）原理，讨论了改性分子筛表面对DNA分子的吸附行为。实验结果表明，功能化的MCM-41可以有效地进行DNA纯化，并且在3-4 mol L−1NaCl溶液中易于释放DNA。本研究可为今后的DNA吸附和富集工作提供一个通用平台。
Three types of MCM-41 absorbents, namely, Al3+–MCM-41, La3+–MCM-41, and Zn2+–MCM-41, were prepared through amine grafting, phosphonate modification, and metal ion chelation and characterized by Fourier transform infrared spectroscopy, thermogravimetric analysis, and N2adsorption–desorption analysis. Results revealed that functionalized MCM-41 maintains the original structure of the molecular sieve and that the pore diameter and surface area are reduced compared with those of pure MCM-41. The adsorption behavior of DNA molecules on the surface of the modified molecular sieves was discussed according to the hard-soft acid–base (HSAB) principle. Experimental results showed that DNA purification could be effectively carried out on functionalized MCM-41 and that DNA is easily released by 3–4 mol L−1NaCl solution. This study could be used as a general platform for future work on DNA adsorption and enrichment.