FECAL SHORT-CHAIN FATTY-ACID (SCFA) ANALYSIS BY CAPILLARY GAS-LIQUID-CHROMATOGRAPHY
FECAL SHORT-CHAIN FATTY-ACID (SCFA) ANALYSIS BY CAPILLARY GAS-LIQUID-CHROMATOGRAPHY
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DOI：
10.1093/ajcn/46.4.641
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发表时间：
1987-10-01
影响因子：
7.1
通讯作者：
KASPER, HW
中科院分区：
文献类型：
--
作者：
SCHEPPACH, WM;FABIAN, CE;KASPER, HW
介绍了一种新的粪便样品中短链脂肪酸（SCFA）分析方法。在通过酸真空转移和通过碱冷冻干燥浓缩的样品纯化后，通过使用毛细管柱的气液色谱法测量SCFAs。峰分辨率和重现性上级传统玻璃柱上的SCFA分析。用这种新技术，定量SCFA在粪便样本中的6名健康志愿者的基础饮食与和不发酵的膳食纤维制剂;纤维对粪便SCFA浓度没有影响。在两名短肠综合征患者中，粪便样本中可发现SCFA，表明发酵活跃。
A new method is described for short-chain fatty acid (SCFA) analysis in stool samples. After sample purification by acid vacuum transfer and concentration by alkaline freeze-drying, SCFAs were measured by gas-liquid chromatography using a capillary column. Peak resolution and reproducibility were superior to SCFA analysis on a conventional glass column. With this new technique SCFA were quantitated in stool samples of six healthy volunteers on a basal diet with and without a fermentable dietary fiber preparation; fiber had no effect on fecal SCFA concentration. In two patients with a short-bowel syndrome, SCFA could be demonstrated in stool samples, indicating active fermentation.