Determination of dexmedetomidine by UHPLC-MS/MS and its application to evaluate the effect of dexmedetomidine concentration on the target-controlled infusion concentration of propofol
Determination of dexmedetomidine by UHPLC-MS/MS and its application to evaluate the effect of dexmedetomidine concentration on the target-controlled infusion concentration of propofol
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UHPLC-MS/MS测定右美托咪定及其在评估右美托咪定浓度对异丙酚靶控输注浓度的影响
DOI：
10.1016/j.jpba.2018.03.015
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发表时间：
2018
影响因子：
3.4
通讯作者：
Li Jiali
中科院分区：
文献类型：
--
作者：
Zheng Zhuoling;Zhang Shuyu;Ma Wudi;Zhang Lingyi;Huang Ling;Huang Wenqi;Huang Min;Wang Zhongxing;Li Jiali
右旋美托咪定(DEX)和异丙酚(PPF)的联合应用在麻醉学领域得到了广泛应用。本研究旨在建立和验证一种快速、简便、灵敏的超高效液相色谱-串联质谱法测定人血浆中地塞米松的方法。该方法用于评价地塞米松浓度对PPF靶控输注(TCI)浓度的影响。色谱柱为Waters XTERRA®MS C18柱，流动相为乙腈-甲醇-含0.1%甲酸和5 mm醋酸铵(70：10：20，v/v/v)的水，流速为0.3minL/ 。采用阳性选择反应监测模式进行质谱分析。监测了地塞米松和替米沙坦的201.12 → 95.12和515.29 → 275.68质量跃迁。地塞米松的线性范围为0.1~10 ng/m L。质控样品批内、批间精密度均小于10.05%，精密度均小于6.25%。新开发的方法被成功地应用于34名患者的血浆样本中地塞米松浓度的定量，这些患者在接受PPF麻醉之前曾服用地塞米松。结果表明，使用DEX可降低PPF的需要量。具体地说，首次发现意识丧失时地塞米松浓度与PPF的TCI浓度呈负相关。
The combination of dexmedetomidine (DEX) and propofol (PPF) is extensively used in the field of anaesthesiology. This study aimed to develop and validate a rapid, simple and sensitive ultra high-performance liquid chromatography-tandem mass spectrometry (UHPLC–MS/MS) method for the determination of DEX in human plasma. The method was applied to evaluate the effect of DEX concentration on the target-controlled infusion (TCI) concentration of PPF. Analytes were separated on a Waters XTERRA®MS C18 column with a mobile phase of acetonitrile-methanol-water containing 0.1% formic acid and 5 mM ammonium acetate (70:10:20,v/v/v) at a flow-rate of 0.3 mL/min. Mass spectrometry was performed in the positive selection reaction monitoring mode. The 201.12 → 95.12 and 515.29 → 275.68 mass transitions of DEX and IS (telmisartan), respectively, were monitored. The calibration curve of DEX was linear over the concentrations of 0.1–10 ng/mL. The intra-batch and inter-batch precisions of quality control samples were less than 10.05% and had accuracies of less than 6.25%. The newly developed method was successfully applied to quantify the DEX concentrations of plasma samples from 34 patients who were co-medicated with DEX prior to receiving anaesthesia by PPF. Results showed that comedication with DEX could reduce the requirements of PPF. Specifically, it was firstly found that the concentration of DEX is negatively correlated with the TCI concentration of PPF at the time of loss of consciousness.