Development of a rapid, simple dipstick dye immunoassay for schistosomiasis diagnosis
Development of a rapid, simple dipstick dye immunoassay for schistosomiasis diagnosis
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DOI：
10.1016/s0022-1759(02)00086-8
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发表时间：
2002-08-01
影响因子：
2.2
通讯作者：
Chao, GQ
中科院分区：
文献类型：
--
作者：
Zhu, YC;He, W;Chao, GQ
为了建立一种快速、简便的血吸虫病免疫诊断方法，将日本血吸虫可溶性虫卵抗原（SEA）与国产蓝色胶体染料（D-1）偶联，用于血吸虫病患者血清抗体的检测。抗原-抗体复合物通过免疫色谱法被吸收到硝酸纤维素膜试纸上的抗人IgG捕获。结果表明，DDIA法检测急性血吸虫病30例（29/30）和慢性血吸虫病84例（79/84）的敏感性分别为96.7%和94.1%。在60例健康受试者中，该检测方法的特异性为96.7%。20例华支睾吸虫和20例并殖吸虫的交叉反应率分别为10.0%和70.0%。结果与常规ELISA检测结果相似。用DDIA试剂盒对121例血吸虫病患者进行现场检测，阳性率为96.7%，环卵沉淀试验（COPT）阳性率为90.1%。与染料缀合的抗原在室温下稳定至少6个月。结果表明，试纸条染料免疫法检测血吸虫病具有灵敏度高、特异性好、快速、简便、价廉、不需要任何仪器设备等优点。与其他免疫检测方法相比，该方法更适用于选择性化疗靶人群的筛选。(C)2002 Elsevier Science B. V.保留所有权利。
In order to develop a rapid, simple immunodiagnostic assay for schistosomiasis, soluble egg antigen (SEA) of Schistosoma japonicum was conjugated with a blue colloidal dye (D-1) produced in China and used to detect antibodies in the sera of schistosomiasis patients. The antigen-antibody complex was captured by anti-human IgG absorbed onto a nitrocellulose membrane dipstick by means of immunochromatography. The results showed that the sensitivity of the dipstick dye immunoassay (DDIA) was 96.7% in 30 cases of acute schistosomiasis (29/30) and 94.1% (79/84) in 84 cases of chronic schistosomiasis. The specificity of the assay was 96.7% in 60 healthy subjects. Cross-reactions were observed in 10.0% of 20 cases of clonorchiasis and in 70.0% of 20 cases of paragonimiasis. The results were similar to those detected by routine ELISA. In a field evaluation of the DDIA kit, the positive rate of the DDIA was 96.7% in 121 cases of schistosomiasis, compared with 90.1% with the circumoval precipitin test (COPT). The antigen conjugated with dye was stable at room temperature for at least 6 months. The results indicated that the dipstick dye immunoassay provided high sensitivity and good specificity for the detection of schistosomiasis and the assay was rapid, simple and cheap, and did not need any equipment. It was more useful for screening target populations for selective chemotherapy than other immunoassays. (C) 2002 Elsevier Science B.V. All rights reserved.