A METHOD FOR MEASURING THE BIODEGRADATION OF ORGANIC-CHEMICALS
A METHOD FOR MEASURING THE BIODEGRADATION OF ORGANIC-CHEMICALS
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DOI：
10.1002/etc.5620050302
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发表时间：
1986-01-01
影响因子：
4.1
通讯作者：
ZIEGLER, DA
中科院分区：
文献类型：
--
作者：
BOATMAN, RJ;CUNNINGHAM, SL;ZIEGLER, DA
描述了一种二氧化碳的自动气相色谱测量方法，并演示了它在生物降解筛选测试程序中的应用。生物降解测试系统由20毫升的玻璃瓶组成，其中包含测试化学品的水溶液和驯化的微生物接种物。瓶子和里面的东西是用橡胶隔板密封的。以指定的时间间隔，对所选小瓶中的内容物进行酸化。随后的二氧化碳顶空自动测定提供了累积生物降解的衡量标准。为了验证整个测试过程的有效性，测试了8种有机化合物的生物降解性：苯胺、苯甲醛、乙二胺四乙酸、乙二醇、直链烷基苯磺酸盐、氮三乙酸、对硝基苯酚和N-甲基苯胺。生物降解也通过测量剩余的测试化学浓度来监测。通过二氧化碳释放值与特定化学分析的比较，结果有很好的内部一致性。二氧化碳测量和特定化学分析的结果都与类似的筛选试验程序得出的文献值有关。从这项研究中得出结论，与现有的测试方法相比，自动测定二氧化碳的顶空有几个优点。进一步的结论是，在所描述的筛选试验中应用这种顶空程序提供了对生物降解性的准确的、尽管保守的估计，积极的结果表明环境持久性的潜力很低。
An automated gas-chromatographic measurement of carbon dioxide is described and its application to a biodegradation screening test procedure is demonstrated. The biodegradation test system consists of 20-ml glass vials containing aqueous solutions of a test chemical and an acclimated microbial inoculum. The vials and their contents are sealed with rubber septa. At specified time intervals, the contents of selected vials are acidified. Subsequent automated headspace determination of carbon dioxide provides a measure of cumulative biological degradation. To demonstrate the validity of the overall test procedure, the biodegradabilities of eight organic compounds were measured: aniline, benzaldehyde, ethylenediamineteraacetic acid, ethylene glycol, linear alkylbenzene sulfonate, nitrilotriacetic acid, p-nitrophenol and N-methylaniline. Biological degradation was also monitored by measuring residual test chemical concentrations. There was excellent internal consistency of results by comparison of carbon dioxide evolution values with specific chemical analyses. Both the results of carbon dioxide measurements and specific chemical analyses are discussed in relation to literature values derived from similar screening test procedures. It is concluded from this study that automated headspace determination of carbon dioxide has several advantages over existing test methods. It is further concluded that application of this headspace procedure in the screening test described provides an accurate, albeit conservative, estimate of biodegradability, with positive results indicating a low potential for environmental persistence.