Current and future prospects for CRISPR-based tools in bacteria.
Current and future prospects for CRISPR-based tools in bacteria.
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DOI：
10.1002/bit.25851
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发表时间：
2016-05
影响因子：
3.8
通讯作者：
Beisel CL
中科院分区：
文献类型：
--
作者：
Luo ML;Leenay RT;Beisel CL
CRISPR-CAS系统已经从耐人寻味的原核生物防御系统迅速过渡到强大而通用的生物分子工具。本文回顾了这些系统如何被转化为操纵细菌遗传学、生理学和群落的技术。最近在细菌中的应用集中在多重基因组编辑、可编程基因调控和序列特异性抗菌素上，而未来的应用可以建立在真核生物的进展、CRISPR-CAS系统丰富的自然多样性以及基于CRISPR的DNA获取的未开发潜力的基础上。总体而言，这些系统已经形成了一个不断扩大的遗传工具箱的基础，并为我们未来对细菌世界的理解和工程提供了巨大的潜力。
CRISPR-Cas systems have rapidly transitioned from intriguing prokaryotic defense systems to powerful and versatile biomolecular tools. This article reviews how these systems have been translated into technologies to manipulate bacterial genetics, physiology, and communities. Recent applications in bacteria have centered on multiplexed genome editing, programmable gene regulation, and sequence-specific antimicrobials, while future applications can build on advances in eukaryotes, the rich natural diversity of CRISPR-Cas systems, and the untapped potential of CRISPR-based DNA acquisition. Overall, these systems have formed the basis of an ever-expanding genetic toolbox and hold tremendous potential for our future understanding and engineering of the bacterial world.