Selective up-regulation of alpha 1-chimaerin mRNA in SK-N-SH neuroblastoma by K+-induced depolarisation
Selective up-regulation of alpha 1-chimaerin mRNA in SK-N-SH neuroblastoma by K+-induced depolarisation
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DOI：
10.1111/j.1432-1033.1996.00820.x
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发表时间：
1996-03-15
期刊：
影响因子：
--
通讯作者：
Lim, L
中科院分区：
文献类型：
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作者：
Dong, JM;Lim, L
编码p21rac的神经元特异性GTP酶激活蛋白的α 1-嵌合蛋白的表达在体内受到空间和时间上的调节。在体外，表达的mRNA的α 1-嵌合蛋白和它的选择性剪接形式，α 2-嵌合蛋白，上调时，人神经母细胞瘤SK-N-SH细胞在无血清培养基中进行神经元型分化。KCl诱导的膜去极化也在转录水平特异性上调SK-N-SH细胞中α 1-嵌合蛋白mRNA的表达。α 1-chimaerin表达的上调膜去极化不是一个立即的早期事件，并发生3小时后KCl治疗。它不需要从头蛋白质合成。作为去极化的结果，通过L型电压敏感性钙通道的钙内流增加是导致α 1-嵌合蛋白mRNA上调的关键事件。还发现α 1-嵌合蛋白表达对高渗性渗透压变化有积极响应。这些结果表明，在体内表达的α 1-嵌合蛋白，一个潜在的信号转导分子，可以调节神经元/突触活动。
The expression of alpha 1-chimaerin, which encodes a neuron-specific GTPase-activating protein for p21rac, is spatially and temporally regulated in vivo. In vitro, expression of the mRNA of both alpha 1-chimaerin and its alternative spliced form, alpha 2-chimaerin, was up-regulated when human neuroblastoma SK-N-SH cells underwent neuronal-type differentiation in a serum-free medium. KCl-induced membrane depolarisation also specifically up-regulated alpha 1-chimaerin mRNA expression in SK-N-SH cells at the transcriptional level. The up-regulation of alpha 1-chimaerin expression by membrane depolarisation is not an immediate early event, and occurs 3 h after KCl treatment. It does not require de novo protein synthesis. The increase in calcium influx via the L-type voltage-sensitive calcium channel as the result of depolarisation is a key event leading to the up-regulation of alpha 1-chimaerin mRNA. alpha 1-Chimaerin expression was also found to respond positively to the hypertonic osmolarity changes. These results suggest that in vivo expression of alpha 1-chimaerin, a potential signal transduction molecule, may be regulated by neuronal/synaptic activity.