A quantitative method for evaluating the degradation of biologic scaffold materials
A quantitative method for evaluating the degradation of biologic scaffold materials
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DOI：
10.1016/j.biomaterials.2006.08.022
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发表时间：
2007-01-01
期刊：
影响因子：
14
通讯作者：
Badylak, Stephen F.
中科院分区：
文献类型：
--
作者：
Gilbert, Thomas W.;Stewart-Akers, Ann M.;Badylak, Stephen F.
源自天然细胞外基质（ECM）的支架已在组织工程和再生医学领域得到广泛应用。许多这些支架被设计为在被新的宿主组织取代时迅速降解。其他支架通过化学交联来减慢降解速度或增加支架的强度。市售的 ECM 支架在组织来源和加工方法方面具有相当大的可变性，并且对其降解速率和降解产物的命运知之甚少。本文描述了一种用放射性同位素 (C-14) 整体标记 ECM 的新方法。研究发现许多组织都被有效标记，包括心脏、肝脏、气管、胰腺、小肠和膀胱组织。在分析的组织中，只有脾脏未发现含有可检测水平的 14C。该技术极其灵敏、准确且安全，但需要使用加速器质谱仪，且昂贵且耗时。该模型代表了第一个描述的定量方法，用于确定 ECM 支架的降解速率并跟踪降解产物的命运。 (c) 2006 Elsevier Ltd. 保留所有权利。
Scaffolds derived from naturally occurring extracellular matrix (ECM) have found extensive use in the fields of tissue engineering and regenerative medicine. Many of these scaffolds are designed to degrade rapidly as they are replaced by new host tissue. Other scaffolds are chemically crosslinked to slow the rate of degradation or add strength to the scaffold. Commercially available ECM scaffolds have considerable variability with regards to tissue origin and methods of processing, and little is known about their rate of degradation and the fate of their degradation products. A novel method is described herein to integrally label ECM with a radioactive isotope (C-14). It was found that a number of tissues are efficiently labeled, including heart, liver, trachea, pancreas, small intestine, and urinary bladder tissue. Of the tissues analyzed, only spleen was not found to contain detectable levels of 14C. The technique is extremely sensitive, accurate, and safe, but requires access to accelerator mass spectrometry, and is expensive and time consuming. This model represents the first described quantitative method to determine the rate of degradation for an ECM scaffold and to track the fate of the degradation products. (c) 2006 Elsevier Ltd. All rights reserved.