Spatial and temporal dynamics of jasmonate synthesis and accumulation in Arabidopsis in response to wounding
Spatial and temporal dynamics of jasmonate synthesis and accumulation in Arabidopsis in response to wounding
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DOI：
10.1074/jbc.m801760200
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发表时间：
2008-06-13
影响因子：
4.8
通讯作者：
Wolfender, Jean-Luc
中科院分区：
文献类型：
--
作者：
Glauser, Gaetan;Grata, Elia;Wolfender, Jean-Luc
采用一种新的代谢产物谱分析方法结合超快速样品制备程序研究了损伤诱导的茉莉酸（JA）及其含氧衍生物在拟南芥中积累的时空动态。除了已知的茉莉酸酯，包括羟基茉莉酸酯（HOJAs）、茉莉单酰基-异亮氨酸（JA-Ile）及其12-羟基衍生物（12-HOJA-Ile）之外，还发现了新的创伤诱导的二羧基茉莉酸酯，12-羧基茉莉单酰基-L-异亮氨酸（12-HOOCJA-Ile）。HOJA和12-HOOCJA-Ile在受伤叶片的中脉中富集，强烈区分它们与所研究的其他茉莉酸代谢物。这些oxlipins在生理pH值的极性与它们的外观在中脉。当确定不同茉莉酸酯积累的时间点时，发现JA水平在创伤后2-5分钟内增加。值得注意的是，这些变化发生在整个植物中，并不局限于受伤的叶子。的速度的刺激，导致JA积累在叶片远端的伤口是至少3厘米/分钟。这些数据提供了新的见解茉莉酸的空间和时间积累，并在理解长距离的伤口信号在植物中的影响。
A new metabolite profiling approach combined with an ultrarapid sample preparation procedure was used to study the temporal and spatial dynamics of the wound-induced accumulation of jasmonic acid (JA) and its oxygenated derivatives in Arabidopsis thaliana. In addition to well known jasmonates, including hydroxyjasmonates (HOJAs), jasmonoyl-isoleucine (JA-Ile), and its 12-hydroxy derivative (12-HOJA-Ile), a new wound-induced dicarboxyjasmonate, 12-carboxyjasmonoyl-L-isoleucine (12-HOOCJA-Ile) was discovered. HOJAs and 12-HOOCJA-Ile were enriched in the midveins of wounded leaves, strongly differentiating them from the other jasmonate metabolites studied. The polarity of these oxylipins at physiological pH correlated with their appearance in midveins. When the time points of accumulation of different jasmonates were determined, JA levels were found to increase within 2-5 min of wounding. Remarkably, these changes occurred throughout the plant and were not restricted to wounded leaves. The speed of the stimulus leading to JA accumulation in leaves distal to a wound is at least 3 cm/min. The data give new insights into the spatial and temporal accumulation of jasmonates and have implications in the understanding of long-distance wound signaling in plants.