Analysis of the formation of flower shapes in wild species and cultivars of tree peony using the MADS-box subfamily gene
Analysis of the formation of flower shapes in wild species and cultivars of tree peony using the MADS-box subfamily gene
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利用MADS-box亚家族基因分析牡丹野生种和栽培种花形的形成
DOI：
10.1016/j.gene.2011.11.008
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发表时间：
2012-02-01
期刊：
影响因子：
3.5
通讯作者：
Zhang, Jingjing
中科院分区：
文献类型：
--
作者：
Shu, Qingyan;Wang, Liangsheng;Zhang, Jingjing
牡丹品种与野生种相比具有独特的特征，雄蕊花瓣化导致花瓣轮生增多，形成不同的花型，是牡丹品种分类的重要特征之一。为了研究花瓣状雄蕊的形成机制，我们从23个牡丹样品中克隆了MADS-box基因家族中B亚家族的PsTM 6基因的编码序列（666 bp）和基因组DNA序列。基因组DNA序列由5个内含子和6个外显子组成。第3、4内含子的顺式调控元件分析表明，它们在所有样品中高度保守。部分推测的氨基酸分析结果表明，PsTM 6旁系同源物的功能分化明显影响雄蕊花瓣状体和花的形状的形成，由于极性和电子电荷的差异引起的氨基酸取代。滑动窗口分析表明，PsTM 6的不同区域受到不同的选择力，特别是在K结构域。这是首次尝试研究基于PsTM 6序列的雄蕊花瓣状体的遗传控制。这将为进一步了解PsTM 6基因的进化及其在牡丹雄蕊形态决定中的作用提供依据。皇冠版权所有（C）2011由Elsevier B. V.出版。保留所有权利。
Tree peony (Paeonia suffricotisa) cultivars have a unique character compared with wild species; the stamen petalody results in increased whorls of petals and generates different flower forms, which are one of the most important traits for cultivar classification. In order to investigate how petaloid stamens are formed, we obtained the coding sequence (666 bp) and genomic DNA sequence of the PsTM6 genes (belongs to B subfamily of MADS-box gene family) from 23 tree peony samples. Five introns and six exons consisted of the genomic DNA sequence. The analysis of cis-acting regulatory elements in the third and fourth intron indicated that they were highly conserved in all samples. Partial putative amino acids were analyzed and the results suggested that functional differentiation of PsTM6 paralogs apparently affected stamen petalody and flower shape formation due to due to amino acid substitution caused by differences in polarity and electronic charge. Sliding window analysis indicated that the different regions of PsTM6 were subjected to different selection forces, especially in the K domain. This is the first attempt to investigate genetic control of the stamen petalody based on the PsTM6 sequence. This will provide a basis for understanding the evolution of PsTM6 and its the function of in determining stamen morphology of tree peony. Crown Copyright (C) 2011 Published by Elsevier B.V. All rights reserved.