Molecular analysis of Drosophila eyes absent mutants reveals features of the conserved Eya domain.
Molecular analysis of Drosophila eyes absent mutants reveals features of the conserved Eya domain.
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发表时间：
2000-06
期刊：
影响因子：
3.3
通讯作者：
Q. T. Bui;J. Zimmerman;H. Liu;N. Bonini
中科院分区：
文献类型：
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作者：
Q. T. Bui;J. Zimmerman;H. Liu;N. Bonini
Eya基因对果蝇眼睛的形成至关重要; Eya功能丧失后，眼睛祖细胞通过程序性细胞死亡而死亡。此外，异位eya表达指导眼睛的形成，并且eya在体内与眼睛发育的另外两个基因sine oculis和dachshund在功能上协同作用，在体外与它们在物理上相互作用。Eya蛋白序列，而高度保守的脊椎动物，是新的。为了确定对Eya蛋白功能至关重要的氨基酸，我们对Eya等位基因进行了测序。这些突变揭示了整个Eya结构域的缺失对于Eya活性是无效的，但是在Eya结构域内具有截短的等位基因显示部分功能。然后，我们将分子遗传分析扩展到Eya结构域内的相互作用。这项分析揭示了对与Sine Oculis或腊肠犬互动特别重要的区域。Eya结构域中的选择性Eya错义突变减少了与Sine Oculis或Dachshund的相互作用。综上所述，这些数据表明，保守的Eya结构域是Eya活性的关键，并可能在它的功能亚区。
The eyes absent (eya) gene is critical to eye formation in Drosophila; upon loss of eya function, eye progenitor cells die by programmed cell death. Moreover, ectopic eya expression directs eye formation, and eya functionally synergizes in vivo and physically interacts in vitro with two other genes of eye development, sine oculis and dachshund. The Eya protein sequence, while highly conserved to vertebrates, is novel. To define amino acids critical to the function of the Eya protein, we have sequenced eya alleles. These mutations have revealed that loss of the entire Eya Domain is null for eya activity, but that alleles with truncations within the Eya Domain display partial function. We then extended the molecular genetic analysis to interactions within the Eya Domain. This analysis has revealed regions of special importance to interaction with Sine Oculis or Dachshund. Select eya missense mutations within the Eya Domain diminished the interactions with Sine Oculis or Dachshund. Taken together, these data suggest that the conserved Eya Domain is critical for eya activity and may have functional subregions within it.