Using radiotelemetry to study behavioural thermoregulation in insects under field conditions
Using radiotelemetry to study behavioural thermoregulation in insects under field conditions
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使用无线电遥测技术研究野外条件下昆虫的行为温度调节
DOI：
10.1111/2041-210x.13251
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发表时间：
2019
影响因子：
6.6
通讯作者：
J. Overgaard
中科院分区：
文献类型：
--
作者：
Christian K. Ørskov;T. Tregenza;J. Overgaard
体温调节是动物生理学的一个重要方面。移动的外温生物有可能通过它们的位置和方向影响它们的温度。行为温度调节已在昆虫中得到广泛研究，特别是在飞蝗中。然而，大多数实地研究仅限于白天观察，通常使用侵入式热电偶，具有明显的破坏自然行为的潜力。我们证明，微型无线电发射器是一种替代和侵入性较小的方法来研究昆虫的温度调节。我们讨论了如何使用这种方法来研究长时间自由放养动物的热行为。具体来说，我们表明，有一个密切的相关性，从植入热电偶在蝗虫L。migratoria和外部安装在同一动物的无线电发射机。我们的实验相匹配的早期观察蝗虫的体温调节行为，确认蝗虫与发射机表现出“正常”的体温调节反应喂养和感染（行为热）。最后，我们通过记录蝗虫在半场环境中的自然温度调节行为来证明基于无线电发射器的系统的实用性。研究表明，蝗虫在白天积极选择温暖的小气候，在夜间积极选择寒冷的小气候。我们的结论是，使用无线电遥测在野生昆虫的行为温度调节的研究可以提供独特的连续记录体温超过几天。这些数据将使研究人员更全面地了解昆虫在自然界中如何使用行为体温调节。
Thermoregulation is a central aspect of animal physiology. Mobile ectotherms have the potential to influence their temperature through their location and orientation. Behavioural thermoregulation has been extensively studied in insects, particularly in the migratory locust Locusta migratoria. However, most field studies are confined to daytime observations typically using invasive thermocouples with obvious potential to disrupt natural behaviour. We demonstrate that miniature radiotransmitters represent an alternative and less invasive method to study insect thermoregulation. We discuss how this method can be used to study the thermal behaviour of free‐ranging animals for extended periods. Specifically, we show that there is a close correlation between temperature recordings from implanted thermocouples in locusts L. migratoria and externally mounted radiotransmitters on the same animals. Our experiments match earlier observations of locust thermoregulatory behaviour confirming that the locusts with transmitters exhibit ‘normal’ thermoregulatory responses to feeding and to infections (behavioural fever). Finally, we demonstrate the practicality of a radiotransmitter‐based system by recording natural thermoregulatory behaviour of locusts in a semi‐field setting. Our field study showed locusts actively chose warm microclimates during the day and cold microclimates at night. We conclude that the use of radiotelemetry in studies of behavioural thermoregulation in wild insects could provide unique continuous recordings of body temperature over several days. Such data will provide researchers with a more complete understanding of how insects use behavioural thermoregulation in nature.