Bombyx mori nucleopolyhedrovirus protein Bm11 is involved in occlusion body production and occlusion-derived virus embedding.
Bombyx mori nucleopolyhedrovirus protein Bm11 is involved in occlusion body production and occlusion-derived virus embedding.
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DOI：
10.1016/j.virol.2018.10.026
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发表时间：
2019-01
期刊：
影响因子：
3.7
通讯作者：
Haiping Wang;Weifan Xu;Xiangshuo Kong;Ying Fan;Xiaofeng Wu
中科院分区：
文献类型：
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作者：
Haiping Wang;Weifan Xu;Xiangshuo Kong;Ying Fan;Xiaofeng Wu
Bombyx morinonuclepolyhedrovirus (BmNPV)orf11（bm11）是一个功能未知的高度保守基因。它与AcMNPVorf19同源。本研究构建了abm11敲除病毒，并对其作用进行了探讨。表达分析表明Bm11是一个晚期基因，共聚焦显微镜分析表明Bm11主要定位于感染晚期的核环区。bm11的缺失不影响芽病毒（BV）的产生或病毒基因组的复制，但显著减少了闭塞体（OBs）的产生和闭塞源病毒（odv）的包埋。生物实验表明Bm11在体内直接注射时参与了BmNPV的感染。总之，我们的研究结果表明，尽管Bm11对BV的产生或成熟ODV的形成不是必需的，但它影响OB的产生和ODV的闭塞。
Bombyx morinucleopolyhedrovirus (BmNPV)orf11(bm11) is a highly conserved gene with unknown function. It is homologous to AcMNPVorf19. In this study, abm11knockout virus was constructed and its role was investigated. Expression analysis indicated thatbm11is a late gene and confocal microscopy analysis demonstrated that Bm11 localizes predominantly in the nuclear ring zone at the late phase of infection. Thebm11deletion did not affect budded virus (BV) production or viral genome replication, but markedly reduced the production of occlusion bodies (OBs) and the embedding of occlusion-derived viruses (ODVs). Bio-assays showed that Bm11 was involved in BmNPV infectivityin vivoby direct injection. In conclusion, our results demonstrated that although Bm11 is not essential for BV production or mature ODV formation, it affects OB production and ODV occlusion.