Sensitivity of photoacoustic microscopy.
Sensitivity of photoacoustic microscopy.
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DOI：
10.1016/j.pacs.2014.04.002
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发表时间：
2014-06
期刊：
影响因子：
7.9
通讯作者：
Wang LV
中科院分区：
文献类型：
--
作者：
Yao J;Wang LV
基于其高空间分辨率，深穿透深度和出色的图像对比度，3D光声显微镜（PAM）自2005年首次出版以来发展迅速。PAM集光激发和声探测于一体，突破了光扩散和光衍射的极限。PAM对光吸收的相对灵敏度为100%（即光吸收系数变化给定百分比和光声振幅变化相同百分比），其最终探测灵敏度仅受热噪声的限制。本文从PAM的工程方面出发，讨论了PAM的检测灵敏度，比较了不同PAM设计的检测效率，总结了各种内源性和外源性造影剂的成像性能。然后介绍了具有高检测灵敏度的典型PAM应用程序，并概述了进一步改进的途径。
Building on its high spatial resolution, deep penetration depth and excellent image contrast, 3D photoacoustic microscopy (PAM) has grown tremendously since its first publication in 2005. Integrating optical excitation and acoustic detection, PAM has broken through both the optical diffusion and optical diffraction limits. PAM has 100% relative sensitivity to optical absorption (i.e., a given percentage change in the optical absorption coefficient yields the same percentage change in the photoacoustic amplitude), and its ultimate detection sensitivity is limited only by thermal noise. Focusing on the engineering aspects of PAM, this Review discusses the detection sensitivity of PAM, compares the detection efficiency of different PAM designs, and summarizes the imaging performance of various endogenous and exogenous contrast agents. It then describes representative PAM applications with high detection sensitivity, and outlines paths to further improvement.