Protocatechuic acid inhibits proliferation, migration and inflammatory response in rheumatoid arthritis fibroblast-like synoviocytes
Protocatechuic acid inhibits proliferation, migration and inflammatory response in rheumatoid arthritis fibroblast-like synoviocytes
复制标题
原儿茶酸抑制类风湿性关节炎成纤维细胞样滑膜细胞的增殖、迁移和炎症反应
DOI：
10.1080/21691401.2020.1776307
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发表时间：
2020-01-01
影响因子：
5.8
通讯作者：
Kong, Lingli
中科院分区：
文献类型：
--
作者：
Wu, Huiqiang;Wang, Jing;Kong, Lingli
类风湿性关节炎（RA）是一种与成纤维细胞样滑膜细胞（FLS）调节异常密切相关的慢性关节炎性疾病。原儿茶酸（PCA）是花青素的酚类化合物，具有抗炎活性。然而，PCA在RA中的作用尚未研究。在本研究中，我们的目的是探讨PCA对RA-FLS的影响。结果表明，PCA可抑制RA-FLS的增殖、侵袭和迁移，并呈剂量依赖性。PCA治疗还可抑制RA-FLS中基质金属蛋白酶（MMP）-3和MMP-13的表达，以及TNF-α、IL-1 β、IL-6等炎性细胞因子的分泌。此外，PCA处理显著诱导RA-FLS细胞凋亡。发现PCA抑制NF-κ B信号传导的活化，这通过p-p65表达的降低和I κ B α表达的增加来证明。PCA可显著降低RA-FLS中Akt和mTOR的磷酸化水平。结果表明，PCA通过抑制NF-κ B和Akt/mTOR信号通路对RA-FLS具有抑制作用。这些发现支持PCA可能是治疗RA的治疗剂的概念。
Rheumatoid arthritis (RA) is a chronic joint inflammatory disease that is closely associated with dysregulation of fibroblast-like synoviocytes (FLSs). Protocatechuic acid (PCA), a phenolic compound of anthocyanins, has been proven to possess anti-inflammatory activity. However, the role of PCA in RA has not been investigated. In the present study, we aimed to explore the effects of PCA on the RA-FLSs. The results showed that PCA suppressed the proliferation, invasion, and migration of RA-FLSs in a dose-dependent manner. PCA treatment also inhibited the expressions of matrix metalloproteinase (MMP)-3 and MMP-13, as well as the secretion of inflammatory cytokines including TNF-alpha, IL-1 beta, IL-6 in RA-FLSs. Moreover, cell apoptosis of RA-FLSs was significantly induced by PCA treatment. PCA was found to repress the activation of NF-kappa B signalling, which was evidenced by the decreased expression of p-p65 and increased expression of I kappa B alpha. Furthermore, PCA significantly decreased the phosphorylation levels of Akt and mTOR in RA-FLSs. In conclusion, the results indicated that PCA exhibited an inhibitory effect on RA-FLSs via inhibiting the NF-kappa B and Akt/mTOR signalling pathways. These findings supported the concept that PCA might be a therapeutic agent for RA treatment.