Knockout of SWS2 in zebrafish (Danio rerio) reveals its roles in feeding and phototactic behaviors
Knockout of SWS2 in zebrafish (Danio rerio) reveals its roles in feeding and phototactic behaviors
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DOI：
10.1016/j.gene.2023.148059
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发表时间：
2023-12-16
期刊：
影响因子：
3.5
通讯作者：
Lu，Ke
中科院分区：
文献类型：
--
作者：
Wang，Yu-Ye;Liang，Xu-Fang;Lu，Ke
脊椎动物的共同祖先有四个视锥细胞视蛋白亚家族来获得色觉：紫外线敏感（SWS 1）、蓝光敏感（SWS 2）、中波长敏感（RH 2）和长波敏感（LWS）。然而，真兽目哺乳动物在夜间生活方式中丢失了SWS 2和RH 2视蛋白。许多研究表明SWS 1和LWS视锥细胞在摄食、择偶和皮肤色素细胞形成中的作用。然而，SWS 2和RH 2锥的作用仍然难以捉摸。在本研究中，我们使用一个理想的模型视觉系统，斑马鱼，它仍然有四个视锥细胞视蛋白，产生SWS 2敲除斑马鱼线。通过各种行为测试，我们发现sws 2-/-斑马鱼幼鱼的摄食量比野生型有所增加。此外，sws 2-/-斑马鱼幼鱼光信号转导途径的基因表达量显著增加。与野生型相比，突变体斑马鱼对红光的趋光性减弱，对黄光、红光和蓝光的敏感性发生改变。但是突变体和野生型斑马鱼都喜欢红光而不是其他波长的光。综上所述，我们认为SWS 2视锥在斑马鱼的摄食和趋光性中不是必需的。
Common ancestor of vertebrates had four cone opsin subfamilies to obtain color vision: ultraviolet-sensitive (SWS1), blue-sensitive (SWS2), middle wavelength sensitive (RH2) and long wavelength sensitive (LWS). Nevertheless, eutherian mammals had lost the SWS2 and RH2 opsins during their nocturnal lifestyle. Many studies had demonstrated the role of SWS1 and LWS cones in feeding, mate choice and skin pigment cell formation. However, the role of SWS2 and RH2 cones remain elusive. In the present study, we used an ideal model visual system, zebrafish, which still have the four cone opsins, to generate a SWS2 knockout zebrafish line. Through various behavioral test, we found thatsws2-/-zebrafish larvae exhibited increased food intake compared with WT. Additionally, there were significantly increased the gene expression of phototransduction pathways insws2-/-zebrafish larvae. Compared to WT, mutant zebrafish showed weaker phototaxis of red light and changed sensitivity of yellow, red and blue lights. But both mutant and WT zebrafish preferred the red light than other wavelengths of light. Taken together, we proposed that SWS2 cone is not necessary for feeding and phototaxis in zebrafish.