Genome-Wide Analyses of the Soybean F-Box Gene Family in Response to Salt Stress.
Genome-Wide Analyses of the Soybean F-Box Gene Family in Response to Salt Stress.
复制标题
大豆 F-Box 基因家族响应盐胁迫的全基因组分析
DOI：
10.3390/ijms18040818
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发表时间：
2017-04-12
影响因子：
5.6
通讯作者：
Lam HM
中科院分区：
文献类型：
--
作者：
Jia Q;Xiao ZX;Wong FL;Sun S;Liang KJ;Lam HM
F-box家族是植物中最大的基因家族之一，它调节包括盐响应在内的多种生命过程。然而，对大豆F-box基因及其在耐盐性中的作用的了解仍然有限。在这里，我们进行了大豆F-box家族的全基因组调查，并通过在线RNA测序（RNA-seq）数据和定量逆转录聚合酶链反应（qRT-PCR）的计算机分析，以预测其潜在的功能，他们的表达分析响应盐度。共鉴定出509个基因编码的725个潜在的F-box蛋白，分为9个亚家族。对基因结构、保守结构域和染色体分布进行了分析。共鉴定出76对重复基因，包括全基因组的节段和串联重复事件，这导致了F-box基因数量的扩增。计算机表达分析表明，这些基因可能参与多种发育功能，并在盐响应中发挥重要作用。我们的qRT-PCR分析证实了12个盐响应F-box基因。总的来说，我们的研究结果提供了有用的信息，大豆F-box基因，特别是它们在耐盐性的潜在作用。
The F-box family is one of the largest gene families in plants that regulate diverse life processes, including salt responses. However, the knowledge of the soybean F-box genes and their roles in salt tolerance remains limited. Here, we conducted a genome-wide survey of the soybean F-box family, and their expression analysis in response to salinity via in silico analysis of online RNA-sequencing (RNA-seq) data and quantitative reverse-transcription polymerase chain reaction (qRT-PCR) to predict their potential functions. A total of 725 potential F-box proteins encoded by 509 genes were identified and classified into 9 subfamilies. The gene structures, conserved domains and chromosomal distributions were characterized. There are 76 pairs of duplicate genes identified, including genome-wide segmental and tandem duplication events, which lead to the expansion of the number of F-box genes. The in silico expression analysis showed that these genes would be involved in diverse developmental functions and play an important role in salt response. Our qRT-PCR analysis confirmed 12 salt-responding F-box genes. Overall, our results provide useful information on soybean F-box genes, especially their potential roles in salt tolerance.