Patient isolates of Borrelia burgdorferi sensu lato with genotypic and phenotypic similarities of strain 25015.
Patient isolates of Borrelia burgdorferi sensu lato with genotypic and phenotypic similarities of strain 25015.
复制标题
与菌株 25015 基因型和表型相似的伯氏疏螺旋体患者分离株。
DOI：
10.1093/infdis/174.5.1112
复制
发表时间：
1996
期刊：
影响因子：
--
通讯作者：
Picken,MM
中科院分区：
文献类型：
--
作者：
Picken,RN;Cheng,Y;Strle,F;Picken,MM
菌株 25015 是一种非典型蜱分离株，属于一般分类群伯氏疏螺旋体 (Borrelia burgdorferisensu lato) 中的一个独特基因组 (DNI27)。据报道，该菌株与伊利诺伊州分离的白足小鼠菌株 CT39 之间有相似之处。隔离过程中 B.在斯洛文尼亚患者培养的 burgdorferisensu lato 中，鉴定出 9 个分离株具有与菌株 25015 和 CT39 相同的遗传特征，基因组 DNA 的限制性内切酶 MluI 消化模式证明了这一点。本研究的目的是对所有 11 个分离株进行分子表征，以检查它们的基因型和表型相似程度。分子研究结果表明患者分离株与菌株 25015 和 CT39 之间存在密切关系。然而，CT39 和一些患者分离株具有反映其离散个体发育的独特特征。
Strain 25015 is an atypical tick isolate that belongs to a distinct genomic group (DNI27) within the general taxonBorrelia burgdorferisensu lato. Similarities between this strain and a white-footed mouse isolate from Illinois, strain CT39, have been reported. In the course of isolatingB. burgdorferisensu lato in culture from Slovenian patients, 9 isolates were identified with the same genetic profiles as strains 25015 and CT39, as evidenced by restriction enzymeMluI digestion patterns of genomic DNA. The aim of the present study was to molecularly characterize all 11 isolates to examine the extent of their genotypic and phenotypic similarity. The results of molecular studies suggest a close relationship between the patient isolates and strains 25015 and CT39. However, CT39 and several patient isolates possessed unique characteristics that reflect their discrete ontogeny.