The role of glutathione S-transferase M1 and T1 gene polymorphisms and fruit and vegetable consumption in antioxidant parameters in healthy subjects.
The role of glutathione S-transferase M1 and T1 gene polymorphisms and fruit and vegetable consumption in antioxidant parameters in healthy subjects.
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DOI：
10.1017/s0007114511003746
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发表时间：
2012-03
期刊：
影响因子：
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通讯作者：
Linhong Yuan;L. Meng;Weiwei Ma;Sheng－li Li;Jin-fang Feng;Huan‐ling Yu;R. Xiao
中科院分区：
文献类型：
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作者：
Linhong Yuan;L. Meng;Weiwei Ma;Sheng－li Li;Jin-fang Feng;Huan‐ling Yu;R. Xiao
谷胱甘肽S转移酶M1/T1基因多态性与氧化应激相关慢性疾病的相关性最近被证实。本研究旨在探讨GSTM1/T1基因多态性与抗氧化性生物标记物及水果蔬菜摄入量(F&V)的关系。在这项研究中，为了进行3d的饮食调查，招募了190名健康成年人。提取DNA后，采用多重聚合酶链式反应方法进行GSTM1/T1基因分型。用分光光度计法测定血浆总抗氧化能力(T-AOC)、维生素C水平和红细胞GST酶活性。用一般线性模型比较不同GSTM1/T1基因型和F&V消耗量的抗氧化参数平均值。GSTM1/T1基因多态性对血浆T-AOC和维生素C水平无影响。GSTM1基因缺失降低了红细胞GST活性。GSTM1型⁻和GSTT1型⁺患者血浆总抗氧化能力与F&V消耗呈正相关。在GSTM1⁻受试者中，红细胞谷胱甘肽转移酶活性也有类似的模式。F&V、GSTM1/T1等位基因的摄入与血浆维生素C水平无相关性。不同的F&V摄入对GSTM1⁻/GSTT1⁺或GSTM1⁻/GSTT1⁻受试者的血浆总抗氧化能力和维生素C水平无影响。在GSTM1⁻/GSTT1⁺基因型者中，红细胞谷胱甘肽转移酶活性对F&V摄入更为敏感。GSTM1/T1基因分型、抗氧化参数与F&V摄入量之间存在关联，需要大规模和多种族的研究来进一步评估这种关系。
The correlation of glutathione S-transferase (GST) M1/T1 genetic polymorphisms with oxidative stress-related chronic diseases was proved recently. The aim of the present study was to investigate the association of GSTM1/T1 genetic polymorphisms with antioxidant biomarkers and consumption of fruits and vegetables (F&V) in healthy subjects. In this study, for conducting a 3 d dietary survey, 190 healthy adults were recruited. After DNA extraction, a multiple PCR method was used for GSTM1/T1 genotyping. A spectrophotometer method was applied for the determination of plasma total antioxidant capacity (T-AOC), vitamin C level and erythrocyte GST enzyme activity. A general linear model was used to compare the mean values of antioxidant parameters for different GSTM1/T1 genotypes and consumption of F&V. Polymorphisms of GSTM1/T1 had no effects on plasma T-AOC and vitamin C levels. Deletion of the GSTM1 gene decreased the erythrocyte GST activity. There was correlation between plasma T-AOC and consumption of F&V in the GSTM1⁻ or GSTT1⁺ subjects. A similar pattern was evident for erythrocyte GST activity in the GSTM1⁻ subjects. No association was found among consumption of F&V and GSTM1/T1 genotypes and plasma vitamin C level. Different consumption of F&V had no impact on plasma T-AOC and vitamin C levels in the GSTM1⁻/GSTT1⁺ or GSTM1⁻/GSTT1⁻ subjects. The erythrocyte GST activity was more sensitive to consumption of F&V in the individuals with the GSTM1⁻/GSTT1⁺ genotype. Association was found among GSTM1/T1 genotypes, antioxidant parameters and consumption of F&V. Large-scale and multiple ethnic studies are needed to further evaluate the relationship.