THE NATURE OF ULTRAVIOLET LIGHT-INDUCED MUTATIONS AT THE HETEROZYGOUS APRT LOCUS IN CHINESE-HAMSTER OVARY CELLS
THE NATURE OF ULTRAVIOLET LIGHT-INDUCED MUTATIONS AT THE HETEROZYGOUS APRT LOCUS IN CHINESE-HAMSTER OVARY CELLS
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DOI：
10.1016/0027-5107(90)90135-q
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发表时间：
1990-10-01
期刊：
影响因子：
--
通讯作者：
GLICKMAN, BW
中科院分区：
文献类型：
--
作者：
DROBETSKY, EA;GLICKMAN, BW
描述了一种用于研究中国仓鼠卵巢（CHO）细胞中杂合基因座处突变特异性的系统。所采用的策略是基于酶促扩增的突变腺嘌呤磷酸核糖转移酶（aprt）等位基因的限制性片段分析和DNA测序。我们已经证明了这种方法的有用性，通过收集的aprt-突变体的特征，在紫外线（UV）诱导的诱变中杂合性丢失事件所发挥的作用。一个类似的策略也被应用到推测的身份负责紫外线诱导的突变热点在aprt基因座的突变前病变。
A system for studying mutational specificity at a heterozygous locus in Chinese hamster ovary (CHO) cells is described. The strategy employed is based on restriction fragment analysis and DNA sequencing of enzymatically amplified mutant adenine phosphoribosyltransferase (aprt) alleles. We have demonstrated the usefulness of this approach through the characterization of a collection of aprt- mutants with respect to the role played by loss of heterozygosity events in ultraviolet light (UV) induced mutagenesis. A similar strategy has also been applied to speculate on the identity of the premutational lesion responsible for a UV-induced mutational hotspot at the aprt locus.