Symbiosis with Francisella tularensis provides resistance to pathogens in the silkworm.
Symbiosis with Francisella tularensis provides resistance to pathogens in the silkworm.
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DOI：
10.1038/srep31476
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发表时间：
2016-08-10
影响因子：
4.6
通讯作者：
Watarai M
中科院分区：
文献类型：
--
作者：
Suzuki J;Uda A;Watanabe K;Shimizu T;Watarai M
土拉热弗朗西丝氏菌是一种高致病力的兼性胞内病原体，广泛存在于动物（包括节肢动物）和环境中。这种细菌已经被发现100多年了，但是F。天然水库中的土拉热病仍基本未知。因此，我们建立了一个新的自然宿主模型。使用家蚕（家蚕）的土拉热病，家蚕是病原体感染的昆虫模型。F.土拉热菌与蚕建立了共生关系，在血淋巴中观察到细菌。感染F. Tularensis，在家蚕中对细菌感染的免疫应答的黑化和黑变的诱导被抑制。用F.土拉热菌增强了抗菌肽的表达和对病原菌感染的抵抗力。这些结果表明，家蚕通过与F. tularensis，这可能对天然水库有重要的健身益处。
Francisella tularensis, the causative agent of tularemia, is a highly virulent facultative intracellular pathogen found in a wide range of animals, including arthropods, and environments. This bacterium has been known for over 100 years, but the lifestyle of F. tularensis in natural reservoirs remains largely unknown. Thus, we established a novel natural host model for F. tularensis using the silkworm (Bombyx mori), which is an insect model for infection by pathogens. F. tularensis established a symbiosis with silkworms, and bacteria were observed in the hemolymph. After infection with F. tularensis, the induction of melanization and nodulation, which are immune responses to bacterial infection, were inhibited in silkworms. Pre-inoculation of silkworms with F. tularensis enhanced the expression of antimicrobial peptides and resistance to infection by pathogenic bacteria. These results suggest that silkworms acquire host resistance via their symbiosis with F. tularensis, which may have important fitness benefits in natural reservoirs.