A SIALIDASE-SUSCEPTIBLE GANGLIOSIDE, IV3-ALPHA(NEUGC-ALPHA-2-8NEUGC)-GG4CER, IS A MAJOR DISIALOGANGLIOSIDE IN WHT/HT MOUSE THYMOMA AND THYMOCYTES
A SIALIDASE-SUSCEPTIBLE GANGLIOSIDE, IV3-ALPHA(NEUGC-ALPHA-2-8NEUGC)-GG4CER, IS A MAJOR DISIALOGANGLIOSIDE IN WHT/HT MOUSE THYMOMA AND THYMOCYTES
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DOI：
10.1093/oxfordjournals.jbchem.a123667
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发表时间：
1991-11-01
影响因子：
2.7
通讯作者：
SUZUKI, A
中科院分区：
文献类型：
--
作者：
NAKAMURA, K;SUZUKI, M;SUZUKI, A
The disialogangliosides of WHT/Ht mouse thymomas, which were obtained by subcutaneous transplantation of a thymoma that developed spontaneously in a WHT/Ht mouse, were purified and characterized. From the results of sugar-composition analysis, a permethylation study, enzymatic hydrolysis followed by TLC-immunostaining, negative-ion fast atom bombardment mass spectrometry (FAB/MS), and H-1 NMR spectroscopy, the structure of one of the five purified disialogangliosides was determined to be IV3-alpha(NeuGc-alpha-2-8NeuGc)-Gg4Cer. The other 4 disialogangliosides were tentatively characterized on the basis of sialidase treatment followed by TLC-immunostaining with cholera toxin B subunit and anti-Gg4Cer antibody to be IV-alpha-(NeuAc-alpha-NeuGc)-Gg4Cer, IV-alpha(NeuGc-alpha-NeuAc)-Gg4Cer, IV-alpha-NeuAc,II3-alpha-NeuAc-Gg4Cer, and IV-alpha-NeuGc,II3-alpha-NeuGc-Gg4Cer. In addition, another component exhibiting one spot on TLC was a mixture of IV-alpha-NeuGc,lI3-alpha-NeuAc-Gg4Cer and IV-alpha-NeuAc,II3-alpha-NeuGc-Gg4Cer. Then the occurrence of these gangliosides in WHT/Ht mouse thymocytes was examined. As one of two major disialogangliosides, the thymocytes contained IV3-alpha(NeuGc-alpha-2-8NeuGc)-Gg4Cer, which was characterized with a mass spectrum and mass chromatograms obtained by micro high-performance liquid chromatography-FAB/MS. The other major disialoganglioside was tentatively characterized to be II3-alpha (NeuGc-alpha-NeuGc)-Gg4Cer by sialidase treatment followed by TLC-immunostaining. A sialidase-susceptible monosialoganglioside, IV3-alpha-NeuGc-Gg4Cer [GM1b(NeuGc)], had been reported to be characteristic of mouse immune tissues [Nakamura, K. et al. (1988) J. Biochem. 103, 201 208]. Taken together, the results suggest that the pathway from Gg4Cer to IV3-alpha-(NeuGc-alpha-2-8NeuGc)-Gg4Cer through GM1b(NeuGc) is quite active in mouse immune tissues.
