Bacillus licheniformis APase I gene promoter: a strong well-regulated promoter in B. subtilis.
Bacillus licheniformis APase I gene promoter: a strong well-regulated promoter in B. subtilis.
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DOI：
10.1099/00221287-137-5-1127
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发表时间：
1991-05
期刊：
影响因子：
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通讯作者：
Jungwan K. Lee;Charles W. Edwards;F. Hulett
中科院分区：
文献类型：
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作者：
Jungwan K. Lee;Charles W. Edwards;F. Hulett
使用从该酶的氨基酸序列推导的合成寡脱氧核苷酸，从地衣芽孢杆菌 MC14 中分离出编码碱性磷酸酶 I (APase I) 氨基末端序列的结构基因的 5' 调节区和部分。该区域的 DNA 序列分析揭示了一个由 129 个氨基酸组成的开放阅读框，其中包含成熟 APase 蛋白的氨基末端序列。蛋白质序列之前是 32 个氨基酸残基的推定信号序列。部分APase克隆的预测氨基酸序列以及实验确定的该酶的氨基酸序列表明，地衣芽孢杆菌APase保留了枯草芽孢杆菌、大肠杆菌、酿酒酵母和各种人体组织的其他APase中保守的重要特征。使用与 lacZ 基因融合的启动子的异源表达研究表明，它在枯草芽孢杆菌中作为非常强的诱导型启动子发挥作用，并受到磷酸盐浓度的严格调节。
The 5' regulatory region and the portion of the structural gene coding for the amino-terminal sequence of alkaline phosphatase I (APase I) were isolated from Bacillus licheniformis MC14 using a synthetic oligodeoxynucleotide deduced from the amino acid sequence of the enzyme. The DNA sequence analysis of this region revealed an open reading frame of 129 amino acids containing the amino-terminal sequence of the mature APase protein. The protein sequence was preceded by a putative signal sequence of 32 amino acid residues. The predicted amino acid sequence of the partial APase clone as well as the experimentally determined amino acid sequence of the enzyme indicated that B. licheniformis APase retains the important features conserved among other APases of Bacillus subtilis, Escherichia coli, Saccharomyces cerevisiae, and various human tissues. Heterologous expression studies of the promoter using a fusion with the lacZ gene indicated that it functions as a very strong inducible promoter in B. subtilis that is tightly regulated by phosphate concentration.