Determination of mono- and disaccharides in milk and milk products by high-performance anion-exchange chromatography with pulsed amperometric detection
Determination of mono- and disaccharides in milk and milk products by high-performance anion-exchange chromatography with pulsed amperometric detection
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DOI：
10.1016/s0003-2670(03)00405-7
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发表时间：
2003-05-26
影响因子：
6.2
通讯作者：
Bianco, G
中科院分区：
文献类型：
--
作者：
Cataldi, TRI;Angelotti, M;Bianco, G
介绍了一种简单、灵敏的液相色谱法，用于分离和定量原料乳和加工乳中的单糖和双糖。在澄清和适当稀释后分析牛奶、水牛、绵羊和山羊乳样品，以定量测定乳糖、半乳糖、葡萄糖和N-乙酰葡糖胺（GlcNAc）。分离是通过高效阴离子交换色谱-脉冲安培检测（HPAEC-PAD）完成的，使用金工作电极和用毫摩尔浓度醋酸钡修饰的稀碱性洗脱液。所采用的洗脱液组合物被设计为提供相对于所选样品的最佳分离，而不受基质组分的干扰。该分析方法成功地用于测定天然存在的单糖和双糖牛奶，马苏里拉奶酪和乳清样品，具有高灵敏度和准确度。(C)2003 Elsevier Science B. V.保留所有权利。
A simple and sensitive liquid chromatographic method for the separation and quantification of mono- and disaccharides in raw- and processed-milk is described. Samples of cows', buffalos', sheeps' and goats' milk were analyzed upon clarification and appropriate dilution for the quantification of lactose, galactose, glucose and N-acetylglucosamine (GlcNAc). The separation was accomplished by high-performance anion-exchange chromatography with pulsed amperometric detection (HPAEC-PAD), using a gold working electrode and dilute alkaline eluents modified by a millimolar concentration of barium acetate. The eluent composition employed was designed to provide optimum separation with respect to the selected sample, without interference from the matrix components. The analytical method was successfully employed for the determination of mono- and disaccharides naturally occurring in dairy milk, mozzarella cheese and whey samples, with high sensitivity and accuracy. (C) 2003 Elsevier Science B.V. All rights reserved.