Inhibitors of protein synthesis also inhibit lysosomal proteolysis. Studies using cystinotic fibroblasts.
Inhibitors of protein synthesis also inhibit lysosomal proteolysis. Studies using cystinotic fibroblasts.
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蛋白质合成抑制剂也抑制溶酶体蛋白水解。
DOI：
10.1172/jci111709
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发表时间：
1985
期刊：
影响因子：
--
通讯作者：
Borysko,K
中科院分区：
文献类型：
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作者：
Thoene,JG;Lemons,R;Boskovich,S;Borysko,K
在无胱氨酸培养基中孵育的胱氨酸耗尽的胱氨酸病成纤维细胞从含胱氨酸的细胞内和细胞外蛋白质的降解中积累无溶酶体的胱氨酸。在这份报告中，我们利用这些细胞的这一特性来研究溶酶体蛋白水解。我们发现，蛋白质合成的抑制剂（放线菌酮，emperazine，和嘌呤霉素）抑制胱氨酸积累内源性蛋白质，因此采取行动，以抑制这些蛋白质的溶酶体蛋白水解。然而，放线菌酮不抑制来源于细胞外富含二硫化物的蛋白质、白蛋白和RNA酶的降解的胱氨酸积累，但放线菌酮抑制来源于胰岛素的溶酶体胱氨酸积累。我们的结论是，细胞内和一些细胞外蛋白质的溶酶体降解可能需要一个快速翻转的蛋白质。图片
Cystine depleted cystinotic fibroblasts incubated in cystine-free medium accumulate lysosomal-free cystine from the degradation of cystine-containing intracellular and extracellular proteins. In this report we have used this characteristic of these cells to study lysosomal proteolysis. We find that inhibitors of protein synthesis (cycloheximide, emetine, and puromycin) inhibit cystine accumulation from endogenous proteins and therefore act to inhibit lysosomal proteolysis of these proteins. However, cycloheximide does not inhibit cystine accumulation derived from the degradation of the extracellular disulfide-rich proteins, albumin and RNase, but lysosomal cystine accumulation derived from insulin is inhibited by cycloheximide. We conclude that a rapidly turning over protein may be required for the lysosomal degradation of intracellular and some extracellular proteins.Images