Effects of tetracycline on the filarial worms Brugia pahangi and Dirofilaria immitis and their bacterial endosymbionts Wolbachia
Effects of tetracycline on the filarial worms Brugia pahangi and Dirofilaria immitis and their bacterial endosymbionts Wolbachia
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DOI：
10.1016/s0020-7519(98)00200-8
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发表时间：
1999-02-01
影响因子：
4
通讯作者：
Sacchi, L
中科院分区：
文献类型：
--
作者：
Bandi, C;McCall, JW;Sacchi, L
沃尔巴克氏体内共生细菌已被证明广泛存在于蠕虫，因此可能在这些线虫的生物学中发挥一定作用。事实上，四环素已被证明可以抑制蠕虫从第三期幼虫的发育，以及感染伯氏丝虫的沙鼠的微丝蚴血症的发展。四环素对沃尔巴克氏体共生菌的抑菌活性可能与这些效应有关，应予以考虑。在这里，我们表明，四环素治疗是非常有效的阻断胚胎发育的两个丝虫，B。pahangi和犬恶丝虫（Dirofilaria immitis）。胚胎退化的TEM记录，而抑制跨卵传播的沃尔巴克氏体的PCR记录。B类沃尔巴克氏体ssrDNA序列的系统发育分析Pahangi证实了细菌内共生体的繁殖与宿主蠕虫的繁殖是一致的。四环素抑制B胚胎发育的可能性。pahangi和D.讨论了细胞质不相容性决定的伊米蒂斯。(C)1999年澳大利亚寄生虫学会。由爱思唯尔科技有限公司出版。保留所有权利。
Wolbachia endosymbiotic bacteria have been shown to be widespread among filarial worms and could thus play some role in the biology of these nematodes. Indeed, tetracycline has been shown to inhibit both the development of adult worms from third-stage larvae and the development of the microfilaraemia in jirds infected with Brugia pahangi. The possibility that these effects are related to the bacteriostatic activity of tetracycline on Wolbachia symbionts should be considered. Here we show that tetracycline treatment is very effective in blocking embryo development in two filarial nematodes, B. pahangi and Dirofilaria immitis. Embryo degeneration was documented by TEM, while the inhibition of the transovarial transmission of Wolbachia was documented by PCR. Phylogenetic analysis on the ssrDNA sequence of the Wolbachia of B. pahangi confirms that the phylogeny of the bacterial endosymbionts is consistent with that of the host worms. The possibility that tetracycline inhibition of embryo development in B. pahangi and D. immitis is determined by cytoplasmic incompatibility is discussed. (C) 1999 Australian Society for Parasitology. Published by Elsevier Science Ltd. All rights reserved.