Expression, purification, and crystallization of a fungal type III polyketide synthase that produces the csypyrones
Expression, purification, and crystallization of a fungal type III polyketide synthase that produces the csypyrones
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产生 csypyrones 的真菌 III 型聚酮合酶的表达、纯化和结晶
DOI：
10.1107/s2053230x14008516
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发表时间：
2014
期刊：
影响因子：
--
通讯作者：
I.
中科院分区：
文献类型：
--
作者：
Yang;D.;Mori;T.;Matsui;T.;Hashimoto;M.;Morita;H.;Fujii;I.;Abe;I.
来自米曲霉的CsyB是一种新型的III型聚酮合成酶，它能催化脂肪酰基辅酶a、丙二酰辅酶a和乙酰乙酰辅酶a生成赛吡酮B1[4-（3-乙酰基-4-羟基-2-氧- 2h -吡喃-6-基）丁酸]。用坐滴蒸汽扩散法对大肠杆菌中表达的重组CsyB进行结晶。晶体属于空间P21，晶胞参数a = 70.0, b = 104.8, c = 73.5 Å， β = 114.4°。
CsyB from Aspergillus oryzae is a novel type III polyketide synthase that catalyzes the formation of csypyrone B1 [4-(3-acetyl-4-hydroxy-2-oxo-2H-pyran-6-yl)butyric acid] from fatty acyl-CoA, malonyl-CoA and acetoacetyl-CoA. Recombinant CsyB expressed in Escherichia coli was crystallized by the sitting-drop vapour-diffusion method. The crystals belonged to space P21, with unit-cell parameters a = 70.0, b = 104.8, c = 73.5 Å, β = 114.4°.