Effect of robotic-assisted treadmill training and chronic quipazine treatment on hindlimb stepping in spinally transected rats
Effect of robotic-assisted treadmill training and chronic quipazine treatment on hindlimb stepping in spinally transected rats
复制标题
DOI：
10.1089/neu.2006.23.1147
复制
发表时间：
2006-07-01
影响因子：
4.2
通讯作者：
Acosta, Cynthia N.
中科院分区：
文献类型：
--
作者：
de Leon, Ray D.;Acosta, Cynthia N.
这项研究的目的是确定机器人辅助跑步机训练是否改善了完全脊髓横断(ST)大鼠的后肢踏步。此外，我们还检查了慢性奎帕津治疗是否会增强机器人辅助训练的有效性。对四组ST大鼠进行后肢踏步实验：训练组+奎帕津、训练组+车辆、非训练组+奎帕津、非训练组+车辆。为了训练老鼠走步，使用了一个机器人设备，在跑步机踏步过程中，后肢以半固定的轨迹移动。这种机器人设备也被用来评估跑步机的踏步。使用鞘内插管将奎帕津或赋形剂注射到腰髓。在ST训练10周后，接受机器人辅助训练的小组在跑步机上进行的踏步动作比未接受训练的小组更多。然而，在ST术后16周，机器人辅助组和未训练组之间没有发现差异。运动学分析显示，所有ST组大鼠都进行了异常的小步幅循环。机器人辅助训练和奎帕嗪联合治疗对步态恢复没有显著影响。这些数据表明，机器人辅助训练可能产生后肢感觉刺激，这在增强腰椎脊髓产生后肢踏步的能力方面是有效的。然而，机器人辅助训练的有效性可能仅限于脊髓横断后恢复的早期阶段。
The purpose of this study was to determine if robotic-assisted treadmill training improved hindlimb stepping in complete spinal cord transected (ST) rats. In addition, we examined whether chronic quipazine treatment would enhance the effectiveness of robotic-assisted training. Hindlimb stepping was examined in four groups of ST rats: trained + quipazine; trained + vehicle; untrained + quipazine; and untrained + vehicle. To train the rats to step, a robotic device was used that moved the hindlimbs in a semi-fixed trajectory during treadmill stepping. The robotic device was also used to assess treadmill stepping. Quipazine or vehicle was administered to the lumbar spinal cord using an intrathecal cannula. The groups that received robotic-assisted training performed more stepping movements on the treadmill than the untrained groups 10 weeks after ST. However, no differences were found between the robotic-assisted and untrained groups 16 weeks after ST. Kinematic analyses revealed that abnormally small step cycles were performed by all of the groups of ST rats. There was no significant effect of combining robotic-assisted training and quipazine treatment on stepping recovery. These data suggest that robotic-assisted training may generate hindlimb sensory stimuli that are effective in enhancing the ability of the lumbar spinal cord to generate hindlimb stepping. However, the effectiveness of robotic-assisted training may be limited to the early stages of recovery following spinal cord transection.