Protective effect of andrographolide against concanavalin A-induced liver injury
Protective effect of andrographolide against concanavalin A-induced liver injury
复制标题
穿心莲内酯对刀豆球蛋白A所致肝损伤的保护作用
DOI：
10.1007/s00210-011-0685-z
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发表时间：
2012-01-01
影响因子：
3.6
通讯作者：
Ning, Guang
中科院分区：
文献类型：
--
作者：
Shi, Guojun;Zhang, Zhijian;Ning, Guang
本实验旨在探讨穿心莲内酯对刀豆素a肝损伤模型的保护作用及其分子机制。结果表明，穿心莲内酯（Ag）可减轻cona诱导的肝损伤，抑制肝细胞凋亡。进一步的结果表明，在Con-A诱导的发病过程中，氧化应激反应基因显著升高。同时，氯化钆和n -乙酰-l-半胱氨酸（NAC）分别灭活Kupffer细胞或减少活性氧，可预防肝损伤。因此我们检测了上述氧化反应基因的信使RNA水平，结果显示Ag处理降低了它们的表达。血清乳酸脱氢酶和髓过氧化物酶活性显著降低。最后，与NAC单独治疗相比，Ag治疗没有进一步降低血清肿瘤坏死因子-α的产生。因此，我们的研究结果表明Ag可以预防con - a诱导的肝损伤，并降低肝脏氧化应激反应。银的肝脏保护作用表明，补充银可能有利于治疗免疫介导的肝损伤。
This study was designed to investigate the hepatic protective effect and the molecular mechanisms of andrographolide in concanavalin A-induced liver injury model. Results showed that andrographolide (Ag) attenuated concanavalin A (Con-A)-induced liver injury and inhibited hepatocyte apoptosis. Further results showed that oxidative stress response genes were significantly elevated during the pathogenesis induced by Con-A. Meanwhile, gadolinium chloride andN-acetyl-l-cysteine (NAC) treatment, which inactivates Kupffer cells or reduces reactive oxygen species, respectively, prevented the liver injury. So the messenger RNA levels of the oxidative response genes mentioned above were detected, and the following results showed that Ag treatment reduced their expression. Besides, serum lactate dehydrogenase and myeloperoxidase activity was significantly reduced by Ag. Finally, Ag treatment did not further reduce serum tumor necrosis factor-α production compared with NAC treatment alone. Thus, our results indicate that Ag prevents Con-A-induced liver injury and reduced the hepatic oxidative stress response. The hepatic protective effect of Ag indicates that Ag supplementation may be beneficial in the treatment of immune-mediated liver injury.