Tomato spotted wilt orthotospovirus influences the reproduction of its insect vector, western flower thrips, Frankliniella occidentalis, to facilitate transmission
Tomato spotted wilt orthotospovirus influences the reproduction of its insect vector, western flower thrips, Frankliniella occidentalis, to facilitate transmission
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番茄斑萎正孢病毒影响其昆虫媒介西花蓟马的繁殖，以促进传播
DOI：
10.1002/ps.5779
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发表时间：
2020
影响因子：
4.1
通讯作者：
Wu QJ
中科院分区：
文献类型：
--
作者：
Wan YR;Hussain S;Merchant A;Xu BY;Xie W;Wang SL;Zhang YJ;Zhou XG;Wu QJ
背景番茄斑萎正背孢病毒（Tomato spotted wilt orthoposovirus，TSWV）是世界范围内观赏植物和蔬菜作物上最具破坏性的病毒之一，由西花蓟马（Frankliniella oceanentalis（Pergande））以持续繁殖的方式传播。TSWV如何影响其媒介的繁殖以增强传播以及TSWV感染是否改变了F.暴露于TSTSWV的蓟马比未暴露的个体具有显著更长的发育时间。更重要的是，发育时间的增加主要与成虫有关，这是传播和病毒传播的关键阶段。此外，TSWV-exposedF.暴露的蓟马比未暴露的蓟马产生更多的后代。有趣的是，后代数量的增加主要来自雄性的增加，雄性具有更大的传播和病毒传播能力。具体而言，后代的雌性/雄性比从1.3-7.0/1变为0.6-1.1/1。至于交配行为，交配时间显着延长TSWV暴露的蓟马。最后，女性在接触病毒时倾向于减少重新交配。重新交配的阻力可能会导致减少精子的可用性在女性，这转化为一个haplodiploid system.CONCLUSIONThese相结合的结果表明，TSWV可以影响发育时间，交配行为，繁殖力和后代性别分配的vectorF。Occupationalism方便病毒传播。因此，能够使用双抗体夹心酶联免疫吸附试验（DAS‐ELISA）、真实的实时定量聚合酶链反应（RT‐qPCR）或病毒检测条早期检测宿主植物和/或其昆虫载体蓟马中的病毒的监测程序可能有利于长期可持续管理。© 2020化学工业学会
BACKGROUNDTomato spotted wilt orthotospovirus(TSWV), one of the most devastating viruses of ornamental plants and vegetable crops worldwide, is transmitted by the western flower thrips,Frankliniella occidentalis(Pergande), in a persistent‐propagative manner. How TSWV influences the reproduction of its vector to enhance transmission and whether infection with TSWV changes the mating behavior ofF. occidentalisare not fully understood.RESULTSTSWV‐exposed thrips had a significantly longer developmental time than non‐exposed individuals. More importantly, increased developmental time was predominantly associated with adults, a stage critical for dispersal and virus transmission. In addition, TSWV‐exposedF. occidentalisproduced substantially more progeny than did non‐exposed thrips. Interestingly, most of the increase in progeny came from an increase in males, a sex with a greater dispersal and virus transmission capability. Specifically, the female/male ratio of progeny shifted from 1.3–7.0/1 to 0.6–1.1/1. As for mating behavior, copulation time was significantly longer in TSWV‐exposed thrips. Finally, females tended to re‐mate less when exposed to the virus. Resistance to re‐mating may lead to reduced sperm availability in females, which translates to a larger number of male progeny under a haplodiploid system.CONCLUSIONThese combined results suggest that TSWV can influence the developmental time, mating behavior, fecundity, and offspring sex allocation of its vectorF. occidentalisto facilitate virus transmission. As such, a monitoring program capable of the earlier detection of the virus in host plants and/or its insect vector, thrips, using double‐antibody sandwich enzyme‐linked immunosorbent assay (DAS‐ELISA), real time quantitative polymerase chain reaction (RT‐qPCR) or virus detection strips might be beneficial for long‐term, sustainable management. © 2020 Society of Chemical Industry