Changes in macrophage and inflammatory cytokine expressions during fracture healing in an ovariectomized mice model.
Changes in macrophage and inflammatory cytokine expressions during fracture healing in an ovariectomized mice model.
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DOI：
10.1186/s12891-021-04360-z
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发表时间：
2021-05-28
影响因子：
2.3
通讯作者：
Shu B
中科院分区：
文献类型：
--
作者：
Chen L;Cheng S;Sun K;Wang J;Liu X;Zhao Y;Yang J;Zhao D;Xue C;Tao Y;Zhao S;Zhang H;Lu S;Shi Q;Wang Y;Shu B
巨噬细胞和炎性细胞因子在骨折愈合中起重要作用。然而，绝经后骨质疏松条件下骨折愈合过程中巨噬细胞和炎性细胞因子的表达模式尚未完全揭示。16周龄雌性小鼠在卵巢摘除或假手术后12 周建立胫骨横断骨折模型。分别于骨折前或骨折后1、3、5、7、14、21、28 取材进行放射学和组织学检查。检测骨折血肿或骨痂中M1/M2炎性巨噬细胞、骨巨噬细胞及肿瘤坏死因子-α、白介素6、白介素1β和巨噬细胞转化相关分子的基因表达。去卵巢小鼠骨折愈合过程延迟，尤其是软骨内骨化和骨痂重塑阶段延迟。骨折血肿或骨痂组织中肿瘤坏死因子-α和白介素6的表达受到干扰，而白介素1β的表达不受影响。肿瘤坏死因子-α的表达在骨折后1、14和21 降低，在骨折后3、5和7天升高。IL-6的表达在第1、3和21DPF时显著升高。我们发现M1和M2巨噬细胞在最初炎症阶段的1DPF时减少。去卵巢小鼠骨折愈合中期14dpf处的M2巨噬细胞和骨折愈合中晚期14dpf、21dpf和28dpf处的骨巨噬细胞也减少。去卵巢小鼠巨噬细胞和炎性细胞因子表达减弱，可能是骨折愈合不良的部分原因。网上版载有补充材料，可在10.1186/s12891-021-04360-z查阅。
Macrophages and inflammatory cytokines play important roles in bone fracture healing. However, the expression patterns of macrophages and inflammatory cytokines during fracture healing under the condition of postmenopausal osteoporosis have not been fully revealed. Tibia transverse fracture was established 12 weeks after ovariectomy or sham operation in 16-week old female mice. Tibias were harvested before fracture or 1, 3, 5, 7, 14, 21, 28 days after fracture for radiological and histological examinations. M1/M2 inflammatory macrophages, osteal macrophages and gene expressions of tumor necrosis factor-α, interleukin-6, interleukin-1β and macrophage conversion related molecules in the fracture haematoma or callus were also detected. The processes of fracture healing, especially the phases of endochondral ossification and callus remodeling, were delayed in ovariectomized mice. The expressions of tumor necrosis factor-α and interleukin-6, but not interleukin-1β, in the fracture haematoma or callus were disturbed. Expressions of tumor necrosis factor-α were decreased at 1, 14 and 21 days post-fracture (DPF), and were increased at 3, 5 and 7 DPF. Interleukin-6 expressions at 1, 3 and 21 DPF were significantly increased. We found the decreases in M1 and M2 macrophages at 1 DPF of the initial inflammatory stage. M2 macrophages at 14 DPF of the middle stage and osteal macrophages at 14, 21 and 28 DPF of the middle and late stages of fracture healing were also reduced in ovariectomized mice. The expressions of macrophages and inflammatory cytokines were impaired in ovariectomized mice, which might contribute partially to poor fracture healing. The online version contains supplementary material available at 10.1186/s12891-021-04360-z.
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影响因子：
2.8
作者：
Lange, Jeffrey;Sapozhnikova, Anna;Lu, Chuanyong;Hu, Diane;Li, Xin;Miclau, Theodore, III;Marcucio, Ralph S.
通讯作者：
Marcucio, Ralph S.
影响因子：
2.8
作者：
Aizawa, T;Kon, T;Gerstenfeld, LC
通讯作者：
Gerstenfeld, LC
影响因子：
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作者：
Alexander, Kylie A.;Chang, Ming K.;Pettit, Allison R.
通讯作者：
Pettit, Allison R.
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影响因子：
4.6
作者：
Feng W;Liu H;Luo T;Liu D;Du J;Sun J;Wang W;Han X;Yang K;Guo J;Amizuka N;Li M
通讯作者：
Li M
DOI：
10.1016/j.jvsv.2016.03.005
发表时间：
2016-10-01
影响因子：
3.2
作者：
Gallagher, Katherine A.;Obi, Andrea T.;Henke, Peter K.
通讯作者：
Henke, Peter K.