Intuitive Label-Free SERS Detection of Bacteria Using Aptamer-Based in Situ Silver Nanoparticles Synthesis
Intuitive Label-Free SERS Detection of Bacteria Using Aptamer-Based in Situ Silver Nanoparticles Synthesis
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DOI：
10.1021/acs.analchem.7b01813
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发表时间：
2017-09-19
影响因子：
7.4
通讯作者：
Xia, Zhiping
中科院分区：
文献类型：
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作者：
Gao, Weicun;Li, Bo;Xia, Zhiping
理想的细菌检测方法应具有灵敏度高、特异性强、操作简便、不需要耗时的培养过程等特点。在这项研究中，我们报告了一种新的细菌检测策略，可以快速，直接识别细菌的表面增强拉曼散射（Sers）形成明确的细菌aptarner @AgNPs。由细菌-适体@AgNPs产生的Sers信号表现出对细菌浓度的线性依赖性（R-2 = 0.9671），范围为10（1）至10（7）cfu/mL。检测限可达1.5cfu/mL。同时，细菌的特异性识别适体可以显著增强细菌的Sers信号，通过Sers光谱可以直接、直观地识别细菌。这种策略消除了以前研究中令人困惑的数据分析，并提供了比现有方法显着的优势，朝着创建基于SERS的生物芯片的关键一步，用于混合物样品中的快速原位细菌检测。
The characteristic of an ideal bacteria-detection method should have high sensitivity and specificity, be easy to operate, and not have a time-consuming culture process. In this study, we report a new bacteria-detection strategy that can recognize bacteria quickly and directly by surface-enhanced Raman scattering (SERS) with the formation of well-defined bacteria-aptarner@AgNPs. SERS signals generated by bacteria-aptamer@AgNPs exhibited a linear dependence on bacteria (R-2 = 0.9671) concentration ranging from 10(1) to 10(7) cfu/mL. The detection limit is sensitive down to 1.5 cfu/mL. Meanwhile, the bacteria SERS signal was dramatically enhanced by its specifically recognized aptamer, and the bacteria could be identified directly and visually through the SERS spectrum. This strategy eliminates the puzzling data analysis of previous studies and offers significant advantages over existing approaches, getting a critical step toward the creation of SERS-based biochips for rapid in situ bacteria detection in mixture samples.