Searching the unknown with gene trapping.
Searching the unknown with gene trapping.
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DOI：
10.1017/s1462399400001824
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发表时间：
2000-07-06
影响因子：
6.2
通讯作者：
Hole, N
中科院分区：
文献类型：
--
作者：
Lako, M;Hole, N
以互补DNA（cDNA）和基因组DNA形式的序列信息的急剧增加在基因的发现和鉴定基因功能的过程之间产生了巨大的差距。为了填补这一空白，“基因捕获”的方法已经开发出来，这结合成一个单一的过程的三个阶段的基因克隆，基因表达模式的研究和相应的突变表型的分析。最近的研究结果表明，基因捕获可以成功地用于克隆参与中枢神经系统，四肢和造血系统发育的特定基因。捕获载体设计的不断改进、cDNA克隆的快速测序和更有效的体外预筛选必将有助于大规模捕获哺乳动物基因组。
dramatic increase in sequence information, both in the form of complementary DNA (cDNA) and genomic DNA, has created a huge gap between the discovery of genes and the process of identifying gene function. To fill this gap, the 'gene-trapping' approach has been developed; this combines into a single process the three stages of gene cloning, the study of the pattern of gene expression and the analysis of the respective mutant phenotype. Recent results indicate that gene trapping can be used successfully to clone specific genes that are involved in the development of the central nervous system, limbs and haematopoietic system. Continuous improvements in the design of trapping vectors, faster sequencing of cDNA clones and more-efficient in vitro pre-screening will certainly aid the large-scale trapping of mammalian genomes.