Protective activities of ribosomal ribonucleic acid and lipopolysaccharide of Pseudomonas aeruginosa: a comparative study
Protective activities of ribosomal ribonucleic acid and lipopolysaccharide of Pseudomonas aeruginosa: a comparative study
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铜绿假单胞菌核糖核酸和脂多糖的保护活性的比较研究
DOI：
10.1007/bf00393676
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发表时间：
1983
期刊：
影响因子：
--
通讯作者：
C. P. V. Boven
中科院分区：
文献类型：
--
作者：
R. Gonggrijp;W. Mullers;C. P. V. Boven
核糖体核糖核酸（RNA）和脂多糖（LPS）。比较了它们对小鼠感染P.铜绿。本研究集中于RNA的保护活性。从纯化的核糖体中分离的RNA不含LPS，如鲎试验所确定的。用二甲基双十八烷基溴化铵（DDA）作佐剂注射RNA，可保护小鼠对抗P。不诱导LPS特异性抗体。对LPS保护活性相对不敏感的C3 H/HeJ小鼠可被RNA保护。研究了突变株的RNA和LPS的保护活性。结果表明，PAC 605的LPS保护活性比PAC IR的LPS保护活性低1000倍，而PAC 605的RNA和PAC IR的LPS保护活性相近。核糖体RNA的保护活性。aerobiosa是非特异性的，因为小鼠也能抵抗异源血清型的P。对大肠杆菌有较好的抑制作用。核糖体RNA。aeruginosa、铜绿假单胞菌E.大肠杆菌和不含脂多糖的酵母菌具有相似的保护活性。用E.大肠杆菌噬菌体MS 2.结论：核糖体RNA具有不同于LPS的保护活性。
Ribosomal ribonucleic acid (RNA) and lipopolysaccharide (LPS) fromP. aeruginosawere compared with respect to their protective activities in mice against an infection withP. aeruginosa. This study is concentrated on the protective activity of RNA. RNA isolated from purified ribosomes did not contain LPS as determined with the Limulus test. Injection of RNA with the adjuvant dimethyldioctadecylammonium bromide (DDA) protected mice againstP. aeruginosawithout inducing LPS-specific antibodies. C3H/HeJ mice which are relatively insensitive to the protective activity of LPS could be protected with RNA. The protective activities of RNA and LPS from a mutant strain ofP. aeruginosa, PAC 605, containing defective lipopolysaccharide, were compared with the protective activities of RNA and LPS from the parent strain, PAC IR. The protective activity of LPS from PAC 605 was 1000 fold lower than the protective activity of LPS from PAC IR. RNA preparations of both strains induced similar percentages of survival. The protective activity of ribosomal RNA fromP. aeruginosawas nonspecific since mice were also protected against a heterologous serotype ofP. aeruginosaand againstEscherichia coli. RNA from ribosomes ofP. aeruginosa, E. coliand the non-lipopolysaccharide containingSaccharomyces cerevisiaehad similar protective activities. No protection was obtained with the ribonucleic acid from theE. coliphage MS 2. It is concluded that ribosomal RNA has protective activities distinct from those of LPS.