Altered hypothalamic signaling and responses to food deprivation in rats fed a low-carbohydrate diet
Altered hypothalamic signaling and responses to food deprivation in rats fed a low-carbohydrate diet
复制标题
DOI：
10.1038/oby.2005.205
复制
发表时间：
2005-10-01
期刊：
影响因子：
--
通讯作者：
Moran, TH
中科院分区：
文献类型：
--
作者：
Kinzig, KP;Scott, KA;Moran, TH
目的：为了模拟低碳水化合物（LC）饮食如何影响食物摄入量和体重，研究方法和程序：在大鼠中监测食物摄入量和体重，所述大鼠获得食物（CH）、LC-高脂肪（HF）或HF饮食。8周后，大鼠接受侧脑室注射黑皮质素激动剂（melanotan-II）和拮抗剂（SHU 9119），并测量摄食反应。处死时，评估血浆激素和下丘脑阿黑皮素原（POMC）、黑皮质素-4受体、神经肽Y（NPY）和促肾上腺皮质激素相关蛋白（AgRP）的mRNA表达。第二组大鼠获得饮食（食物或LC-HF）4周，随后24小时的食物剥夺的两个场合，之后的食物摄入量和下丘脑POMC，神经肽Y，和AgRP mRNA的表达进行了measured.Results：HF大鼠消耗更多的食物和增加更多的体重比大鼠CH或LC-HF饮食。尽管相似的摄入量和体重增加，LC-HF大鼠增加肥胖相对于CH大鼠。LC-HF大鼠对美拉诺坦-II更敏感，对SHU 9119不太敏感。LC-HF大鼠血浆瘦素和ghrelin水平升高，胰岛素水平降低，NPY和POMC mRNA表达模式与食物剥夺大鼠一致。LC-HF大鼠并没有表现出反弹后的食物剥夺，神经肽Y，POMC，和AgRP mRNA的表达水平没有受到影响的deprivation.Discussion：我们的研究结果表明，LC饮食影响多个系统参与控制的食物摄入量和体重。这些数据还表明，LC-HF饮食的维持通过减少对食物剥夺的补偿反应来影响食物摄入。
Objective: To model how consuming a low-carbohydrate (LC) diet influences food intake and body weight.Research Methods and Procedures: Food intake and body weight were monitored in rats with access to chow (CH), LC-high-fat (HF), or HF diets. After,8 weeks, rats received intracerebroventricular injections of a melanocortin agonist (melanotan-II) and antagonist (SHU9119), and feeding responses were measured. At sacrifice, plasma hormones and hypothalamic expression of mRNA for proopiomelanocortin (POMC), melanocortin-4 receptor, neuropeptide Y (NPY), and agouti related protein (AgRP) were assessed. A second set of rats had access to diet (chow or LC-HF) for 4 weeks followed by 24 h food deprivation on two occasions, after which food intake and hypothalamic POMC, NPY, and AgRP mRNA expression were measured.Results: HF rats consumed more food and gained more weight than rats on CH or LC-HF diets. Despite similar intakes and weight gains, LC-HF rats had increased adiposity relative to CH rats. LC-HF rats were more sensitive to melanotan-II and less sensitive to SHU9119. LC-HF rats had increased plasma leptin and ghrelin levels and decreased insulin levels, and patterns of NPY and POMC mRNA expression were consistent with those of food-deprived rats. LC-HF rats did not show rebound hyperphagia after food deprivation, and levels NPY, POMC, and AgRP mRNA expression were not affected by deprivation.Discussion: Our results demonstrate that an LC diet influences multiple systems involved in the controls of food intake and body weight. These data also suggest that maintenance on an LC-HF diet affects food intake by reducing compensatory responses to food deprivation.